BMRB Entry 6933

Title:
Complete Chemical Shift Assignment for the Neurabin PDZ Domain
Deposition date:
2006-01-06
Original release date:
2007-04-11
Authors:
Dancheck, Barbara; Peti, Wolfgang
Citation:

Citation: Kelker, M.; Dancheck, Barbara; Ju, T.; Kessler, R.; Hudak, J.; Nairn, A.; Peti, Wolfgang. "Structural basis for spinophilin-neurabin receptor interaction."  Biochemistry. 46, 2333-2344 (2007).
PubMed: 17279777

Assembly members:

Assembly members:
Neurabin PDZ Domain, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts369
15N chemical shifts99
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurabin PDZ1

Entities:

Entity 1, Neurabin PDZ 94 residues - Formula weight is not available

1   GLYHISMETGLULEUPHEPROVALGLULEU
2   GLULYSASPGLUASPGLYLEUGLYILESER
3   ILEILEGLYMETGLYVALGLYALAASPALA
4   GLYLEUGLULYSLEUGLYILEPHEVALLYS
5   THRVALTHRGLUGLYGLYALAALAGLNARG
6   ASPGLYARGILEGLNVALASNASPGLNILE
7   VALGLUVALASPGLYILESERLEUVALGLY
8   VALTHRGLNASNPHEALAALATHRVALLEU
9   ARGASNTHRLYSGLYASNVALARGPHEVAL
10   ILEGLYARGGLU

Samples:

sample_1: Neurabin PDZ Domain 2 mM; Na phosphate 20 mM; NaCl 50 mM

sample_2: Neurabin PDZ Domain, [U-99% 15N], 3.5 mM; Na phosphate 20 mM; NaCl 50 mM

sample_3: Neurabin PDZ Domain, [U-99% 15N; U-99% 13C], 3.8 mM; Na phosphate 20 mM; NaCl 50 mM

conditions_1: ionic strength: 20 mM; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
CbCaCoNHnot availablenot availableconditions_1
HNCaCbnot availablenot availableconditions_1
HbHaCoNHnot availablenot availableconditions_1
HCCH Tocsynot availablenot availableconditions_1
1H15N NOESYnot availablenot availableconditions_1
HNConot availablenot availableconditions_1

Software:

CARA v1.3.2, Cara - peak assignment

TOPSPIN v1.3, Bruker - processing spectra

ATHNOS-CANDID v1, . - automated peak picking

NMR spectrometers:

  • Bruker AVANCE-DRX 600 MHz
  • Bruker Avance II 500 MHz

Related Database Links:

BMRB 11202
PDB
DBJ BAA86536 BAC77245 BAE22449 BAE23620
GB AAC53454 AAH53748 AAI18112 AAI30450 AAI44110
REF NP_001069621 NP_001159632 NP_001159633 NP_001159634 NP_001159635
SP O35867 Q9ULJ8
TPG DAA30807
AlphaFold O35867 Q9ULJ8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks