BMRB Entry 7035

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for 2nd SH3 domain of human NCK2 adaptor protein
Deposition date:
2006-03-29
Original release date:
2007-09-24
Authors:
Liu, Jingxian
Citation:

Citation: Liu, Jingxian; Li, Minfen; Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "Structural Insight into the Binding Diversity between the Human Nck2 SH3 Domains and Proline-Rich Proteins"  Biochemistry 45, 7171-7184 (2006).
PubMed: 16752908

Assembly members:

Assembly members:
NCK2 2nd SH3, polymer, 57 residues, 6559 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NCK2 2nd SH3: IPAFVKFAYVAEREDELSLV KGSRVTVMEKCSDGWWRGSY NGQIGWFPSNYVLEEVD

Data sets:
Data typeCount
13C chemical shifts99
15N chemical shifts53
1H chemical shifts311
T1 relaxation values63
T2 relaxation values63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NCK2 2nd SH31

Entities:

Entity 1, NCK2 2nd SH3 57 residues - 6559 Da.

1   ILEPROALAPHEVALLYSPHEALATYRVAL
2   ALAGLUARGGLUASPGLULEUSERLEUVAL
3   LYSGLYSERARGVALTHRVALMETGLULYS
4   CYSSERASPGLYTRPTRPARGGLYSERTYR
5   ASNGLYGLNILEGLYTRPPHEPROSERASN
6   TYRVALLEUGLUGLUVALASP

Samples:

sample_1: NCK2 2nd SH3, [U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %

sample_2: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 70 ± 2 %

sample_3: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
HSQC-TOCSYsample_1not availableconditions_1
HSQC-NOESYsample_1not availableconditions_1
HNCACB/CBCANHsample_3not availableconditions_1
HCCH TOCSY/HCCH NOESYnot availablenot availableconditions_1
T1/T2/HetNOEnot availablenot availableconditions_1

Software:

NMRPipe - raw data processing

NMRView - peak assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI47129
GB AAC04831 AAC80284 AAH00103 AAH07195 AAY24332
REF NP_001004720 NP_003572 XP_001109452 XP_001109500 XP_001488099
SP O43639
AlphaFold O43639

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks