BMRB Entry 7054

Title:
NMR chemical shift assignments and structure determination of Xanthomonas campestris XCC1710: Northeast Structural Genomics Consortium Target XcR35
Deposition date:
2006-04-05
Original release date:
2006-04-24
Authors:
Cort, John; Montelione, Gaetano; Ramelot, Theresa; Kennedy, Michael
Citation:

Citation: Cort, John; Montelione, Gaetano; Ramelot, Theresa; Kennedy, Michael. "NMR chemical shift assignments of Xanthomonas campestris XCC1710"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
XCC1710, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Xanthomonas campestris   Taxonomy ID: 339   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Xanthomonas campestris

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts468
15N chemical shifts107
1H chemical shifts751

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XCC17101

Entities:

Entity 1, XCC1710 132 residues - Formula weight is not available

1   METPROLEUASNGLNGLUHISPROASPTYR
2   THRTYRALALEUARGALAALAASPGLYARG
3   HISALALYSVALASNGLUGLNILELEUGLN
4   GLNSERPHEILELEUMETPROASPGLULEU
5   VALGLUHISTRPPROVALPROSERLEUGLY
6   GLNLEUGLNPROALAHISMETASPALAVAL
7   LEUALALEUASNPROALAVALILELEULEU
8   GLYTHRGLYGLUARGGLNGLNPHEPROSER
9   THRASPVALLEUALAALACYSLEUTHRARG
10   GLYILEGLYLEUGLUALAMETTHRASNALA
11   ALAALAALAARGTHRTYRASNVALLEUALA
12   SERGLUGLYARGARGVALALALEUALAMET
13   ILEVALGLYGLYLEUGLUHISHISHISHIS
14   HISHIS

Samples:

sample_1: XCC1710, [U-13C; U-15N], 0.5 ± 0.1 mM; NaCl 100 ± 5 mM; CaCl2 20 ± 2 mM; DTT 10 ± 1 mM; NaN3 0.02 ± 0.002 w/v %; D2O 5 ± .02 v/v %

sample_2: XCC1710, [U-13C; U-15N], 0.5 ± 0.1 mM; NaCl 100 ± 5 mM; CaCl2 20 ± 2 mM; DTT 10 ± 1 mM; NaN3 0.02 ± 0.002 w/v %; D2O 100 ± .02 v/v %

conditions_1: pH: 6.5; pressure: 1000 mbar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
1H13C HSQCnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
HC-TOCSY-NHnot availablenot availableconditions_1
CC-TOCSY-NHnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1

Software:

VNMR - NMR_data_collection

FELIX - processing

SPARKY - analysis

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Unity Plus 500 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
EMBL CAP51909
GB AAM41004 AAY49571 AEL06839
REF NP_637080 WP_011036887 WP_014507482 YP_001903954 YP_005636957

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks