BMRB Entry 7083

Title:
Chemical shifts of SBD from Rhizopu oryzae glucoamylase
Deposition date:
2006-04-24
Original release date:
2007-05-02
Authors:
Liu, Yu-Nan; Lyu, Ping-Chaing
Citation:

Citation: Liu, Yu-Nan; Lai, Yen-Ting; Chou, Wei-I; Chang, Margaret; Lyu, Ping-Chiang. "Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase."  Biochem. J. 403, 21-30 (2007).
PubMed: 17117925

Assembly members:

Assembly members:
SBD, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rhizopus oryzae   Taxonomy ID: 64495   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Rhizopus oryzae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts120
1H chemical shifts639

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SBD1

Entities:

Entity 1, SBD 106 residues - Formula weight is not available

1   ALASERILEPROSERSERALASERVALGLN
2   LEUASPSERTYRASNTYRASPGLYSERTHR
3   PHESERGLYLYSILETYRVALLYSASNILE
4   ALATYRSERLYSLYSVALTHRVALVALTYR
5   ALAASPGLYSERASPASNTRPASNASNASN
6   GLYASNILEILEALAALASERPHESERGLY
7   PROILESERGLYSERASNTYRGLUTYRTRP
8   THRPHESERALASERVALLYSGLYILELYS
9   GLUPHETYRILELYSTYRGLUVALSERGLY
10   LYSTHRTYRTYRASPASNASNASNSERALA
11   ASNTYRGLNVALSERTHR

Samples:

sample_1: SBD 1 mM

sample_2: SBD, [U-98% 15N], 1 mM

sample_3: SBD, [U-98% 15N; U-98% 13C], 1 mM

conditions_1: ionic strength: 10 mM; pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
1H_2DNOESYnot availablenot availableconditions_1
1H_2D_TOCSYnot availablenot availableconditions_1

Software:

SPARKY v3.111 -

NMR spectrometers:

  • bruker avance 600 MHz

Related Database Links:

PDB
DBJ BAH09876 BAH09877 BAH09878
GB ABB77799 AFG20192

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks