BMRB Entry 7158

Title:
Molecular characterization of the Ran binding zinc finger domain
Deposition date:
2006-06-07
Original release date:
2007-04-19
Authors:
Alam, Steven; Higa, Meda; Ullman, Katharine; Sundquist, Wesley
Citation:

Citation: Higa, Meda; Alam, Steven; Sundquist, Wesley; Ullman, Katharine. "Molecular characterization of the ran-binding zinc finger domain of Nup153"  J. Biol. Chem. 282, 17090-17100 (2007).
PubMed: 17426026

Assembly members:

Assembly members:
hNUP153, polymer, 32 residues, 3488 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hNUP153: GHMVIGTWDCDTCLVQNKPE AIKCVACETPKP

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts30
1H chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hNUP153 Zinc finger1
2ZINC (II) ION, 12

Entities:

Entity 1, hNUP153 Zinc finger 32 residues - 3488 Da.

Numbering starting at V3 represents V722 in the hNUP153 protein

1   GLYHISMETVALILEGLYTHRTRPASPCYS
2   ASPTHRCYSLEUVALGLNASNLYSPROGLU
3   ALAILELYSCYSVALALACYSGLUTHRPRO
4   LYSPRO

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: hNUP153, [U-95% 15N], 1.5 ± 0.1 mM; tris buffer 20 ± 0.01 mM; NaCl 50 ± 0.01 mM; BME 5 ± 0.01 mM; ZnCl2 10 ± 0.01 um

sample_2: hNUP153, [U-95% 13C; U-95% 15N], 1.0 ± 0.1 mM; tris buffer 20 ± 0.01 mM; NaCl 50 ± 0.01 mM; BME 5 ± 0.01 mM; ZnCl2 10 ± 0.01 micromolar

conditions_1: ionic strength: 70 mM; pH*: 7.0; pressure: 1.0 atm; temperature: 293 K

conditions_2: ionic strength: 70 mM; pH: 7.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availablenot available
1H13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HC(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCAC(CO)NHnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HN(CO)HBnot availablenot availablenot available
LRCCnot availablenot availablenot available
1H13C 3D-NOESYnot availablenot availablenot available
1H15N 3D NOESYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

CYANA v2.1, CYANA - noe assignment, refinement

VNMR v3.1C, Varian - Aquisition of Data

SPARKY v3.110, Sparky - Assignments

FELIX v2004, Accelrys -

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks