BMRB Entry 7232

Title:
Trans-(4-aminomethyl)phenylazobenzoic acid-aPP bound to DPC micelles
Deposition date:
2006-07-14
Original release date:
2006-10-19
Authors:
Jurt, S.; Aemissegger, A.; Guentert, P.; Zerbe, O.; Hilvert, D.
Citation:

Citation: Jurt, S.; Aemissegger, A.; Guentert, P.; Zerbe, O.; Hilvert, D.. "A Photoswitchable Miniprotein based on the Sequence of Avian Pancreatic Polypeptide"  Angew Chem. Int. Ed. Engl. 45, 6297-6300 (2006).
PubMed: 16933352

Assembly members:

Assembly members:
Pancreatic hormone, 1, polymer, 9 residues, Formula weight is not available
Pancreatic hormone, 2, polymer, 25 residues, Formula weight is not available
EAB, non-polymer, 269.299 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pancreatic hormone, 1: GPSQPTYPG
Pancreatic hormone, 2: PVEDLIRFYNDLQQYLNVVT RHRYX

Data typeCount
1H chemical shifts251

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pancreatic hormone, 11
2Pancreatic hormone, 22
3(3-{(E)-[3-(AMINOMETHYL)PHENYL]DIAZENYL}PHENYL)ACETIC ACID3

Entities:

Entity 1, Pancreatic hormone, 1 9 residues - Formula weight is not available

1   GLYPROSERGLNPROTHRTYRPROGLY

Entity 2, Pancreatic hormone, 2 25 residues - Formula weight is not available

1   PROVALGLUASPLEUILEARGPHETYRASN
2   ASPLEUGLNGLNTYRLEUASNVALVALTHR
3   ARGHISARGTYRNH2

Entity 3, (3-{(E)-[3-(AMINOMETHYL)PHENYL]DIAZENYL}PHENYL)ACETIC ACID - C15 H15 N3 O2 - 269.299 Da.

1   EAB

Samples:

sample_1: Pancreatic hormone, 1 2 mM; Pancreatic hormone, 2 2 mM; (3-{(E)-[3-(AMINOMETHYL)PHENYL]DIAZENYL}PHENYL)ACETIC ACID 2 mM; DPC 300 mM; MES buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50.0 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1

Software:

CYANA v2.2.2 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz