BMRB Entry 7330

Title:
Solution Structure of BmKalphaIT01, an alpha-insect toxin from the Venom of the Chinese Scorpion Buthus martensi Karsch
Deposition date:
2006-11-08
Original release date:
2007-10-09
Authors:
Tong, X.; Chen, X.; Wu, H.
Citation:

Citation: Tong, X.; Zhu, J.; Ma, Y.; Chen, X.; Wu, G.; He, F.; Cao, C.; Wu, H.. "Solution Structure of BmKalphaIT01, an alpha-insect toxin from the Venom of the Chinese Scorpion Buthus martensi Karsch"  Biochemistry 46, 11322-11330 (2007).
PubMed: 17877370

Assembly members:

Assembly members:
Alpha-neurotoxin_TX12, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chinese armor-tail scorpion   Taxonomy ID: 34649   Superkingdom: not available   Kingdom: Eukaryota   Genus/species: Metazoa Mesobuthus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alpha-neurotoxin_TX12: VRDAYIAQNYNCVYHCARDA YCNELCTKNGAKSGSCPYLG EHKFACYCKDLPDNVPIRVP GKCH

Data sets:
Data typeCount
1H chemical shifts428

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha-neurotoxin_TX121

Entities:

Entity 1, Alpha-neurotoxin_TX12 64 residues - Formula weight is not available

1   VALARGASPALATYRILEALAGLNASNTYR
2   ASNCYSVALTYRHISCYSALAARGASPALA
3   TYRCYSASNGLULEUCYSTHRLYSASNGLY
4   ALALYSSERGLYSERCYSPROTYRLEUGLY
5   GLUHISLYSPHEALACYSTYRCYSLYSASP
6   LEUPROASPASNVALPROILEARGVALPRO
7   GLYLYSCYSHIS

Samples:

sample_1: Alpha-neurotoxin_TX12, none, 1.4 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_2: Alpha-neurotoxin_TX12, none, 1.4 mM; phosphate buffer 20 mM; D2O 100%

sample_conditions_1: ionic strength: . .; pH: 4.9; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
DQF-COSYsample_1isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
2D TOCSYsample_2isotropicsample_conditions_1
2D TOCSYsample_2isotropicsample_conditions_1

Software:

VNMR v6.1B, Carlisle, M., Steele, D., Miller, M. - collection, processing

XEASY v1994, Xia, T.-H., Bartel, C. - data analysis

AMBER v5.0, Kollman, P.A., Case, D., Merz, K., Cheatham, T., Simmerling, C., Pharmaceuticals, V. - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAG39640 AAV88456 AAV88457
SP Q9GQW3
AlphaFold Q9GQW3