BMRB Entry 10122

Title:
Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)
Deposition date:
2007-04-02
Original release date:
2008-09-02
Authors:
Nameki, N.; Koshiba, S.; Tochio, N.; Kobayashi, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Nameki, N.; Koshiba, S.; Tochio, N.; Kobayashi, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P021015-57

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts313
15N chemical shifts78
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Intersectin 21

Entities:

Entity 1, Intersectin 2 74 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALGLUASN
2   LEULYSALAGLNALALEUCYSSERTRPTHR
3   ALALYSLYSASPASNHISLEUASNPHESER
4   LYSHISASPILEILETHRVALLEUGLUGLN
5   GLNGLUASNTRPTRPPHEGLYGLUVALHIS
6   GLYGLYARGGLYTRPPHEPROLYSSERTYR
7   VALLYSILEILEPROGLYSERGLUSERGLY
8   PROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.5 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.771, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Guntert, P. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_007110328

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks