BMRB Entry 11321

Name: Solution Structure of the IBR domain of the RING finger protein 31 protein
Published: 2011-08-19

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PDB ID: 2ct7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11321
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the IBR domain of the RING finger protein 31 protein

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Koshiba, S.; Tomizawa, T.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Koshiba, S.; Tomizawa, T.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the IBR domain of the RING finger protein 31 protein" .

Assembly members:

Assembly members:
IBR domain, polymer, 86 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040816-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IBR domain: GSSGSSGALFHKKLTEGVLM RDPKFLWCAQCSFGFIYERE QLEATCPQCHQTFCVRCKRQ WEEQHRGRSCEDFQNWKRMN SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	355
15N chemical shifts	80
1H chemical shifts	540

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IBR domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, IBR domain 86 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

LEU

PHE

2

HIS

LYS

LEU

THR

GLU

GLY

VAL

LEU

MET

3

ARG

ASP

PRO

LYS

PHE

LEU

TRP

CYS

ALA

GLN

4

CYS

SER

PHE

GLY

PHE

ILE

TYR

GLU

ARG

GLU

5

GLN

LEU

GLU

ALA

THR

CYS

PRO

GLN

CYS

HIS

6

GLN

THR

PHE

CYS

VAL

ARG

CYS

LYS

ARG

GLN

7

TRP

GLU

GLN

HIS

ARG

GLY

ARG

SER

CYS

8

GLU

ASP

PHE

GLN

ASN

TRP

LYS

ARG

MET

ASN

9

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: IBR domain, [U-13C; U-15N], 1.55 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CT7
DBJ	BAB15675 BAB70948 BAB84970 BAF35583 BAF83936
GB	AAH09821 AAH12077 AAH17376 AAP12522 AIC60278
REF	NP_001297261 NP_060469 XP_001112195 XP_001166671 XP_001490713
SP	Q96EP0
AlphaFold	Q96EP0