BMRB Entry 15188

Title:
backbone and side chain methly order parameters and correlation times for calcium-saturated calmodulin
Deposition date:
2007-03-19
Original release date:
2007-03-19
Authors:
Frederick, Kendra; Marlow, Michael; Valentine, Kathleen; Wand, Joshua
Citation:

Citation: Frederick, Kendra; Marlow, Michael; Valentine, Kathleen; Wand, Joshua. "Conformational entropy in molecular recognition by proteins"  Nature 448, 325-329 (2007).
PubMed: 17637663

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data typeCount
order parameters134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLN
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Samples:

sample_1: calmodulin, [U-100% 15N], 1 mM; potassium chloride 100 mM; imidazole 20 mM; CaCl2 6 mM; sodium azide 3 mM

sample_2: calmodulin, [U-100% 13C; U-100% 15N; 55% 2H], 1 mM; potassium chloride 100 mM; imidazole 20 mM; CaCl2 6 mM; sodium azide 3 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15191 15470 15624 15650 15852 16418 16465 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0608335A
REF NP_001008160 NP_001009759 NP_001039714 NP_001040234 NP_001080864
SP O16305 P02594 P21251 P62144 P62145
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
AlphaFold P21251 P62145 P62144 P02594 O16305