BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15440

Title: 1H, 13C and 15N resonance assignments of the C-terminal domain of HasB from Serratia marcescens   PubMed: 19636864

Authors: Lefevre, Julien; Simenel, Catherine; Delepelaire, Philippe; Delepierre, Muriel; Izadi-Pruneyre, Nadia

Citation: Lefevre, Julien; Simenel, Catherine; Delepelaire, Philippe; Delepierre, Muriel; Izadi-Pruneyre, Nadia. "(1)H, (13)C and (15)N resonance assignments of the C-terminal domain of HasB, a specific TonB like protein, from Serratia marcescens"  Biomol. NMR Assignments 1, 197-199 (2007).

Assembly members:
HasB, polymer, 132 residues, Formula weight is not available

Natural source:   Common Name: Bacillus marcescens   Taxonomy ID: 615   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Serratia marcescens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HasB: MKVQEQSVGAPPPGRADKTA APQTRLTPYAQAGEDNWRSR ISGRLNRFKRYPKDALRLKR QGVGQVRFTLDRQGHVLAVT LVSSAGLPSLDREIQALVKR ASPLPTPPADAYVNGTVELT LPIDFSLRGAGF

Data sets:
Data typeCount
13C chemical shifts546
15N chemical shifts125
1H chemical shifts905

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HasB1

Entities:

Entity 1, HasB 132 residues - Formula weight is not available

HasB is the periplasmic domain of a membrane protein

1   METLYSVALGLNGLUGLNSERVALGLYALA
2   PROPROPROGLYARGALAASPLYSTHRALA
3   ALAPROGLNTHRARGLEUTHRPROTYRALA
4   GLNALAGLYGLUASPASNTRPARGSERARG
5   ILESERGLYARGLEUASNARGPHELYSARG
6   TYRPROLYSASPALALEUARGLEULYSARG
7   GLNGLYVALGLYGLNVALARGPHETHRLEU
8   ASPARGGLNGLYHISVALLEUALAVALTHR
9   LEUVALSERSERALAGLYLEUPROSERLEU
10   ASPARGGLUILEGLNALALEUVALLYSARG
11   ALASERPROLEUPROTHRPROPROALAASP
12   ALATYRVALASNGLYTHRVALGLULEUTHR
13   LEUPROILEASPPHESERLEUARGGLYALA
14   GLYPHE

Samples:

sample_1: HasB, [U-99% 13C; U-99% 15N], 0.8 mM; Sodium Phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAE46937 CDG10879
GB AGE16788 AIA50123 ALD43843 ALL36754 EMF03481
REF WP_004940473 WP_015376815 WP_016928895 WP_021504696 WP_025301729