BMRB Entry 15449

Title:
Type I rat fatty acid synthase acyl carrier protein (ACP) domain
Deposition date:
2007-08-30
Original release date:
2008-01-28
Authors:
Ploskon, E.; Arthur, C.; Evans, S.; Williams, C.; Crosby, J.; Crump, M.
Citation:

Citation: Ploskon, E.; Arthur, C.; Evans, S.; Williams, C.; Crosby, J.; Simpson, T.; Crump, M.. "A Mammalian Type I fatty acid synthase acyl carrier protein domain does not bind acyl chains"  J. Biol. Chem. 283, 518-528 (2008).
PubMed: 17971456

Assembly members:

Assembly members:
Fatty_acid_synthase_(EC_2.3.1.85), polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts88
1H chemical shifts609

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 89 residues - Formula weight is not available

1   GLYASPGLYGLUALAGLNARGASPLEUVAL
2   LYSALAVALALAHISILELEUGLYILEARG
3   ASPLEUALAGLYILEASNLEUASPSERSER
4   LEUALAASPLEUGLYLEUASPSERLEUMET
5   GLYVALGLUVALARGGLNILELEUGLUARG
6   GLUHISASPLEUVALLEUPROILEARGGLU
7   VALARGGLNLEUTHRLEUARGLYSLEUGLN
8   GLUMETSERSERLYSALAGLYSERASPTHR
9   GLULEUALAALAPROLYSSERLYSASN

Samples:

sample: ACP1 – 2 mM; sodium deuteroacetate 20 mM; sodium azide 1 mM; DTT 1 mM; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsampleisotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

ANALYSIS, CCPN - data analysis

ARIA v1.2, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA31780 CAA31882 CAA44679 CAA44680
GB AAA41144 AAA41145 AAA57219 EDM06908 EDM06909
REF NP_059028
SP P12785
AlphaFold P12785

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks