BMRB Entry 15508

Title:
Chemical shift values for the innermost lipoyl domain of E. coli PDH complex
Deposition date:
2007-10-04
Original release date:
2007-10-12
Authors:
Dafydd, Jones
Citation:

Citation: Jones, Dafydd; Stott, Katherine; Howard, Mark; Perham, Richard. "Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli"  Biochemistry 39, 8448-8459 (2000).
PubMed: 10913250

Assembly members:

Assembly members:
lipoyl_domain, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11c

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E2plip1

Entities:

Entity 1, E2plip 79 residues - Formula weight is not available

1   VALLYSGLUVALASNVALPROASPILEGLY
2   GLYASPGLUVALGLUVALTHRGLUVALMET
3   VALLYSVALGLYASPLYSVALALAALAGLU
4   GLNSERLEUILETHRVALGLUGLYASPLYS
5   ALASERMETGLUVALPROALAPROPHEALA
6   GLYVALVALLYSGLULEULYSVALASNVAL
7   GLYASPLYSVALLYSTHRGLYSERLEUILE
8   METILEPHEGLUVALGLUGLYALAALA

Samples:

sample_1: lipoyl domain, [U-100% 15N], 2-3 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

  • Bruker AM 500 MHz

Related Database Links:

PDB
EMBL CSG13687 CSG32591 CSG39424
GB EFI22553 EFZ46147 EGB41640 EGB60459 EGI12521
REF WP_000295776 WP_000374927 WP_032230881 WP_050541395

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks