BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16071

Title: Mouse prion protein fragment 90-231 at pH 7.0   PubMed: 19546219

Authors: Damberger, Fred; Wuthrich, Kurt; Hornemann, Simone; von Schroetter, Christine

Citation: Hornemann, Simone; von Schroetter, Christine; Damberger, Fred; Wuthrich, Kurt. "Prion protein-detergent micelle interactions studied by NMR in solution."  J. Biol. Chem. 284, 22713-22721 (2009).

Assembly members:
mPrP90, polymer, 144 residues, 164114.1 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mPrP90: GSGQGGGTHNQWNKPSKPKT NLKHVAGAAAAGAVVGGLGG YMLGSAMSRPMIHFGNDWED RYYRENMYRYPNQVYYRPVD QYSNQNNFVHDCVNITIKQH TVTTTTKGENFTETDVKMME RVVEQMCVTQYQKESQAYYD GRRS

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts127
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mPrP901

Entities:

Entity 1, mPrP90 144 residues - 164114.1 Da.

1   GLYSERGLYGLNGLYGLYGLYTHRHISASN
2   GLNTRPASNLYSPROSERLYSPROLYSTHR
3   ASNLEULYSHISVALALAGLYALAALAALA
4   ALAGLYALAVALVALGLYGLYLEUGLYGLY
5   TYRMETLEUGLYSERALAMETSERARGPRO
6   METILEHISPHEGLYASNASPTRPGLUASP
7   ARGTYRTYRARGGLUASNMETTYRARGTYR
8   PROASNGLNVALTYRTYRARGPROVALASP
9   GLNTYRSERASNGLNASNASNPHEVALHIS
10   ASPCYSVALASNILETHRILELYSGLNHIS
11   THRVALTHRTHRTHRTHRLYSGLYGLUASN
12   PHETHRGLUTHRASPVALLYSMETMETGLU
13   ARGVALVALGLUGLNMETCYSVALTHRGLN
14   TYRGLNLYSGLUSERGLNALATYRTYRASP
15   GLYARGARGSER

Samples:

sample_1: mPrP90, [U-99% 13C; U-99% 15N], 1.2 ± 0.05 mM; H2O 90%; D2O 10%; Sodium Phosphate buffer at pH 7.0 5 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 7.0; pressure: 1 atm; temperature: 293.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

GB AAA39997