BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16104

Title: THE DYNAMIC ALPHA-HELIX STRUCTURE OF MICELLE-BOUND HUMAN AMYLIN.   PubMed: 19244249

Authors: Patil, Sharadrao; Xu, Shihao; Sheftic, Sarah; Alexandrescu, Andrei

Citation: Patil, Sharadrao; Xu, Shihao; Sheftic, Sarah; Alexandrescu, Andrei. "THE DYNAMIC ALPHA-HELIX STRUCTURE OF MICELLE-BOUND HUMAN AMYLIN."  J. Biol. Chem. 284, 11982-11991 (2009).

Assembly members:
alpha-helix, polymer, 37 residues, 3909.330 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha-helix: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
15N chemical shifts44
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-helix1

Entities:

Entity 1, alpha-helix 37 residues - 3909.330 Da.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Samples:

sample_2: entity, [U-100% 15N], 0.5 mM; sodium dodecyl sulfate, [U-99% 2H], 100 mM; acetic acid, [U-99% 2H], 60 mM

sample_1: entity, [U-99% 15N], 0.5 mM; SDS, [U-2H], 100 mM; acetic acid 60 mM

sample_conditions_1: ionic strength: 60 mM; pH: 4.6; pressure: 1.0 atm; temperature: 310 K

sample_conditions_2: ionic strength: 60 mM; pH*: 4.2; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D TROSYsample_1isotropicsample_conditions_1
3D TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.851, Brunger - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16105 17394 18795 20045
PDB
DBJ BAG73319
EMBL CAA33032 CAA37002 CAA39504 CAA48724 CAB57803
GB AAA35524 AAA35983 AAA51728 AAA52281 AAI11850
REF NP_000406 XP_001144800 XP_003265632 XP_003265633 XP_003828947
SP P10997