BMRB Entry 16671

Title:
Mechanism for the selective interaction of C-terminal EH-domain proteins with specific NPF-containing partners
Deposition date:
2010-01-11
Original release date:
2010-02-23
Authors:
KIEKEN, Fabien; SHARMA, Mahak; JOVIC, Marko; PANAPAKKAM GIRIDHARAN, Sai Srnivas; NASLAVSKY, Naava; CAPLAN, Steve; SORGEN, Paul
Citation:

Citation: Kieken, Fabien; Sharma, Mahak; Jovic, Marko; Giridharan, Sai Srinivas Panapakkam; Naslavsky, Naava; Caplan, Steve; Sorgen, Paul. "Mechanism for the Selective Interaction of C-terminal Eps15 Homology Domain Proteins with Specific Asn-Pro-Phe-containing Partners."  J. Biol. Chem. 285, 8687-8694 (2010).
PubMed: 20106972

Assembly members:

Assembly members:
EH_domain_of_EHD1, polymer, 105 residues, 11654.459 Da.
MICAL_L1_like_peptide, polymer, 15 residues, 1419.530 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGex6p-2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts365
15N chemical shifts92
1H chemical shifts823

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EH_domain_of_EHD11
2MICAL_L1_like_peptide2
3Calcium ion3

Entities:

Entity 1, EH_domain_of_EHD1 105 residues - 11654.459 Da.

1   GLYPROLEUGLYSERASPASPVALGLUTRP
2   VALVALGLYLYSASPLYSPROTHRTYRASP
3   GLUILEPHETYRTHRLEUSERPROVALASN
4   GLYLYSILETHRGLYALAASNALALYSLYS
5   GLUMETVALLYSSERLYSLEUPROASNTHR
6   VALLEUGLYLYSILETRPLYSLEUALAASP
7   VALASPLYSASPGLYLEULEUASPASPGLU
8   GLUPHEALALEUALAASNHISLEUILELYS
9   VALLYSLEUGLUGLYHISGLULEUPROALA
10   ASPLEUPROPROHISLEUVALPROPROSER
11   LYSARGARGHISGLU

Entity 2, MICAL_L1_like_peptide 15 residues - 1419.530 Da.

1   LEUGLUSERLYSPROTYRASNPROPHEGLU
2   GLUGLUGLUGLUASP

Entity 3, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EH domain of EHD1, [U-99% 13C; U-99% 15N], 0.6 mM; MICAL L1 like peptide 2 mM; Tris 20 mM; Kcl 100 mM; Cac12 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 13CFiltered-13CfilteredNOESYsample_1isotropicsample_conditions_1
2D 13C-filtered-15N editedNOESYsample_1isotropicsample_conditions_1
2D 15N filtered Noesysample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ProcheckNMR, Laskowski and MacArthur - data analysis

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15279 16179 16180 16181
PDB
DBJ BAB28540 BAC40684 BAE32742 BAE35499 BAE35852
EMBL CAH90816
GB AAB81204 AAD45423 AAF24223 AAG02009 AAH37094
REF NP_001011939 NP_001015578 NP_001125465 NP_001162473 NP_001248124
SP Q5E9R3 Q5RBP4 Q641Z6 Q9H4M9 Q9WVK4
AlphaFold Q9UH45 Q5E9R3 Q9H4M9 Q641Z6 Q5RBP4 Q9WVK4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks