BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16904

Title: Backbone assignment of human alpha-synuclein in viscous 2 M glucose solution   PubMed: 20872101

Authors: Wu, Kuen-Phon; Baum, Jean

Citation: Wu, Kuen-Phon; Baum, Jean. "Backbone assignment and dynamics of human -synuclein in viscous 2M glucose solution."  Biomol. NMR Assignments 5, 43-46 (2011).

Assembly members:
alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts135
1H chemical shifts134
T2 relaxation values129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

13C-15N: alpha-synuclein, [U-100% 13C; U-100% 15N], 0.3 – 0.4 mM; H20 80%; D20 20%; glucose 2 M; phosphate 10 mM; NaCl 138 mM; KCl 2 mM

15N: alpha-synuclein, [U-100% 15N], 0.1 – 0.2 mM; H20 80%; D20 20%; glucose 2 M; phosphate 10 mM; NaCl 138 mM; KCl 2 mM

2_M_glucose_in_PBS: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15Nisotropic2_M_glucose_in_PBS
3D CBCA(CO)NH13C-15Nisotropic2_M_glucose_in_PBS
3D HNCO13C-15Nisotropic2_M_glucose_in_PBS
3D HNCACB13C-15Nisotropic2_M_glucose_in_PBS
3D HN(CA)CO13C-15Nisotropic2_M_glucose_in_PBS
2D 1H-15N HSQC15Nisotropic2_M_glucose_in_PBS
3D HNN13C-15Nisotropic2_M_glucose_in_PBS
T215Nisotropic2_M_glucose_in_PBS

Software:

VNMRJ v2.1, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAB29375 BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143