BMRB Entry 16939

Title:
WT alpha-synuclein fibrils
Deposition date:
2010-05-19
Original release date:
2011-07-07
Authors:
Comellas, Gemma; Kloepper, Kathryn; Nieuwkoop, Andrew; Lemkau, Luisel; Ladror, Daniel; Ebisu, Reika; Woods, Wendy; Lipton, Andrew; George, Julia; Rienstra, Chad
Citation:

Citation: Comellas, Gemma; Lemkau, Luisel; Nieuwkoop, Andrew; Kloepper, Kathryn; Ladror, Daniel; Ebisu, Reika; Woods, Wendy; Lipton, Andrew; George, Julia; Rienstra, Chad. "Structured Regions of alpha-Synuclein Fibrils Include the Early-Onset Parkinson's Disease Mutation Sites."  J. Mol. Biol. 411, 881-895 (2011).
PubMed: 21718702

Assembly members:

Assembly members:
WT_alpha-synuclein_fibrils, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: peT28

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha synuclein protein1

Entities:

Entity 1, alpha synuclein protein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

U-13C_15N: WT alpha-synuclein fibrils, [U-13C; U-15N], mM; H2O 100%

2-glycerol_sample: WT alpha-synuclein fibrils, [2-glycerol] 13-C, U-15N, mM; H2O 100%

1_3-glycerol_sample: WT alpha-synuclein fibrils, [1,3-glycerol] 13C, U-15N, mM; H2O 100%

U-13C_15N_doubling_material: WT alpha-synuclein fibrils, [U-13C; U-15N], mM; H2O 100%

sample_conditions_1: pH: 7.5; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
NCACX 50 ms DARRU-13C_15Nisotropicsample_conditions_1
NCOCX 50 ms DARRU-13C_15Nisotropicsample_conditions_1
NCOCX 250 ms DARRU-13C_15Nisotropicsample_conditions_1
CANcoCX 50 ms DARRU-13C_15Nisotropicsample_conditions_1
CONcaCX 10 ms DARRU-13C_15Nisotropicsample_conditions_1
NCACX 250 ms DARR2-glycerol_sampleisotropicsample_conditions_1
NCOCX 250 ms DARR2-glycerol_sampleisotropicsample_conditions_1
NCOCX 250 ms DARR1_3-glycerol_sampleisotropicsample_conditions_1
CANCO2-glycerol_sampleisotropicsample_conditions_1
CANCO1_3-glycerol_sampleisotropicsample_conditions_1
NCACX 50 ms DARRU-13C_15N_doubling_materialisotropicsample_conditions_1
NCOCX 50 ms DARRU-13C_15N_doubling_materialisotropicsample_conditions_1
NCACX 500 ms DARR2-glycerol_sampleisotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143
AlphaFold P37840 P61139 P61140 P61142 P61143