BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17095

Title: Identification by NMR of the Binding Surface for the Histidine-Containing Phosphocarrier Protein HPr on the N-Terminal Domain of Enzyme of the Escherichia coli Phophotransferase System   PubMed: 9109646

Authors: Garrett, Daniel; Seok, Yeong-Jae; Peterkofsky, Alan; Clore, G.; Gronenborn, Angela

Citation: Garrett, Daniel; Seok, Yeong-Jae; Peterkofsky, Alan; Clore, G.; Gronenborn, Angela. "Identification by NMR of the Binding Surface for the Histidine-Containing Phosphocarrier Protein HPr on the N-Terminal Domain of Enzyme I of the Escherichia coli Phosphotransferase System"  Biochemistry 36, 4393-4398 (1997).

Assembly members:
EIN, polymer, 259 residues, Formula weight is not available
HPr, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
EIN: MISGILASPGIAFGKALLLK EDEIVIDRKKISADQVDQEV ERFLSGRAKASAQLETIKTK AGETFGEEKEAIFEGHIMLL EDEELEQEIIALIKDKHMTA DAAAHEVIEGQASALEELDD EYLKERAADVRDIGKRLLRN ILGLKIIDLSAIQDEVILVA ADLTPSETAQLNLKKVLGFI TDAGGRTSHTSIMARSLELP AIVGTGSVTSQVKNDDYLIL DAVNNQVYVNPTNEVIDKMR AVQEQVASEKAELAKLKDR
HPr: MFQQEVTITAPNGLHTRPAA QFVKEAKGFTSEITVTSNGK SASAKSLFKLQTLGLTQGTV VTISAEGEDEQKAVEHLVKL MAELE

Data sets:
Data typeCount
binding constants1
kinetic rates2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EIN1
2HPr2

Entities:

Entity 1, EIN 259 residues - Formula weight is not available

1   METILESERGLYILELEUALASERPROGLY
2   ILEALAPHEGLYLYSALALEULEULEULYS
3   GLUASPGLUILEVALILEASPARGLYSLYS
4   ILESERALAASPGLNVALASPGLNGLUVAL
5   GLUARGPHELEUSERGLYARGALALYSALA
6   SERALAGLNLEUGLUTHRILELYSTHRLYS
7   ALAGLYGLUTHRPHEGLYGLUGLULYSGLU
8   ALAILEPHEGLUGLYHISILEMETLEULEU
9   GLUASPGLUGLULEUGLUGLNGLUILEILE
10   ALALEUILELYSASPLYSHISMETTHRALA
11   ASPALAALAALAHISGLUVALILEGLUGLY
12   GLNALASERALALEUGLUGLULEUASPASP
13   GLUTYRLEULYSGLUARGALAALAASPVAL
14   ARGASPILEGLYLYSARGLEULEUARGASN
15   ILELEUGLYLEULYSILEILEASPLEUSER
16   ALAILEGLNASPGLUVALILELEUVALALA
17   ALAASPLEUTHRPROSERGLUTHRALAGLN
18   LEUASNLEULYSLYSVALLEUGLYPHEILE
19   THRASPALAGLYGLYARGTHRSERHISTHR
20   SERILEMETALAARGSERLEUGLULEUPRO
21   ALAILEVALGLYTHRGLYSERVALTHRSER
22   GLNVALLYSASNASPASPTYRLEUILELEU
23   ASPALAVALASNASNGLNVALTYRVALASN
24   PROTHRASNGLUVALILEASPLYSMETARG
25   ALAVALGLNGLUGLNVALALASERGLULYS
26   ALAGLULEUALALYSLEULYSASPARG

Entity 2, HPr 85 residues - Formula weight is not available

1   METPHEGLNGLNGLUVALTHRILETHRALA
2   PROASNGLYLEUHISTHRARGPROALAALA
3   GLNPHEVALLYSGLUALALYSGLYPHETHR
4   SERGLUILETHRVALTHRSERASNGLYLYS
5   SERALASERALALYSSERLEUPHELYSLEU
6   GLNTHRLEUGLYLEUTHRGLNGLYTHRVAL
7   VALTHRILESERALAGLUGLYGLUASPGLU
8   GLNLYSALAVALGLUHISLEUVALLYSLEU
9   METALAGLULEUGLU

Samples:

sample_1: EIN, [U-15N], 1 mM; HPr, [U-15N], 1.6 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_2: EIN, [U-15N], 1 mM; sodium phospahte 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.1; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

CAPP, Garrett - data analysis

STAPP, (Garrett et al., 1991) - data analysis

FRODO, (Jones, 1978) - docking study

GRASP, (Nicholls et al., 1991) - docking study

RIBBONS, (Carson, 1987) - visualization

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 19958 25731 4106 4264 17274 18379 2060 2371 29 4264
PDB
DBJ BAA16290 BAB36711 BAG78231 BAI26669 BAI31701 BAA16289 BAB36710 BAB92987 BAG78230 BAH64556
EMBL CAP76888 CAQ32794 CAQ88296 CAQ99314 CAR03878 CAA32865 CAA35818 CAD07663 CAG73805 CAH21953
GB AAA24385 AAA24441 AAC75469 AAG57535 AAN43978 AAA23655 AAA24384 AAA24438 AAA24440 AAA27052
REF NP_311315 NP_416911 NP_708271 WP_000112669 WP_000328837 NP_288977 NP_311314 NP_416910 NP_456968 NP_461366
SP P08839 P0AA04 P0AA05 P0AA06 P0AA07 P0AA08
PIR AE0810
PRF 1107231B