BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17099

Title: 1H NMR Studies of the Binding of EDTA to Bovine Pancreatic Ribonuclease   PubMed: 416852

Authors: Brauer, Manfred; Benz, F.

Citation: Brauer, Manfred; Benz, F.. "1H NMR Studies of the Binding of EDTA to Bovine Pancreatic Ribonuclease"  Biochim. Biophys. Acta 533, 186-194 (1978).

Assembly members:
ribonuclease, polymer, 124 residues, 292.243 Da.
{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID, non-polymer, 292.243 Da.

Natural source:   Common Name: Cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metaoza   Genus/species: Bos taurus

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
ribonuclease: KETAAAKFERQHMDSSTSAA SSSNYCNQMMKSRNLTKDRC KPVNTFVHESLADVQAVCSQ KNVACKNGQTNCYQSYSTMS ITDCRETGSSKYPNCAYKTT QANKHIIVACEGNPYVPVHF DASV

Data sets:
  • assigned_chemical_shifts
  • binding_constants
  • pKa_values
Data typeCount
binding constants1
pKa values4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ribonuclease1
2EDTA2

Entities:

Entity 1, ribonuclease 124 residues - 292.243 Da.

1   LYSGLUTHRALAALAALALYSPHEGLUARG
2   GLNHISMETASPSERSERTHRSERALAALA
3   SERSERSERASNTYRCYSASNGLNMETMET
4   LYSSERARGASNLEUTHRLYSASPARGCYS
5   LYSPROVALASNTHRPHEVALHISGLUSER
6   LEUALAASPVALGLNALAVALCYSSERGLN
7   LYSASNVALALACYSLYSASNGLYGLNTHR
8   ASNCYSTYRGLNSERTYRSERTHRMETSER
9   ILETHRASPCYSARGGLUTHRGLYSERSER
10   LYSTYRPROASNCYSALATYRLYSTHRTHR
11   GLNALAASNLYSHISILEILEVALALACYS
12   GLUGLYASNPROTYRVALPROVALHISPHE
13   ASPALASERVAL

Entity 2, EDTA - C10 H16 N2 O8 - 292.243 Da.

1   EDT

Samples:

sample_1: deuteroacetate buffer 0.2 M; ribonuclease 2.0 mM; EDTA0 – 63.4 mM; D2O 100%

sample_2: deuterocetate buffer 0.2 M; ribonuclease 2.0 mM; EDTA 63.4 mM; D2O 100%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 307 K

Experiments:

NameSampleSample stateSample conditions
1H NMRsample_1isotropicsample_conditions_1
1H NMRsample_2isotropicsample_conditions_1

Software:

none_given, na - na

NMR spectrometers:

  • Bruker WH270 270 MHz

Related Database Links:

BMRB 1072 16010 16011 16503 16742 17172 19065 2928 385 4031 4032 443
PDB
EMBL CAA30263 CAA33801 CAB37066
GB AAA72269 AAA72757 AAB35594 AAB36134 AAI49530
PIR JC5560 NRBOB
PRF 630436A
REF NP_001014408 XP_005211519 XP_005901936 XP_010837737
SP P61823 P61824
TPE CDG32088
TPG DAA25470