BMRB Entry 17424

Title:
Solution NMR Structure of Transmembrane and Cytosolic Regions of Integrin 1 in Detergent Micelles
Deposition date:
2011-01-24
Original release date:
2012-08-14
Authors:
Lai, Chaohua; Liu, Xiaoxi; Tian, Changlin
Citation:

Citation: Lai, Chaohua; Liu, Xiaoxi; Tian, Changlin; Wu, Fangming. "Integrin 1 has a long helix, extending from the transmembrane region to the cytoplasmic tail in detergent micelles."  PLoS ONE 8, e62954-e62954 (2013).
PubMed: 23646163

Assembly members:

Assembly members:
Integrin_Alpha1, polymer, 54 residues, 5272.820 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Integrin_Alpha1: MGLPGRVPLWVILLSAFAGL LLLMLLILALWKIGFFKRPL KKKMEKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts176
15N chemical shifts38
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Integrin_Alpha11

Entities:

Entity 1, Integrin_Alpha1 54 residues - 5272.820 Da.

1   METGLYLEUPROGLYARGVALPROLEUTRP
2   VALILELEULEUSERALAPHEALAGLYLEU
3   LEULEULEUMETLEULEUILELEUALALEU
4   TRPLYSILEGLYPHEPHELYSARGPROLEU
5   LYSLYSLYSMETGLULYSLEUGLUHISHIS
6   HISHISHISHIS

Samples:

15N-labeled: sodium phosphate 50 mM; DTT 2 mM; Integrin Alpha 1, [U-15N], 1.0 mM; H20 95%; D20 5%

15N-13C-labeled: Integrin Alpha1, [U-13C; U-15N], 1.0 mM; sodium phosphate 50 mM; DTT 2 mM; H20 90%; D20 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N-labeledisotropicsample_conditions_1
2D 1H-15N HSQC15N-13C-labeledisotropicsample_conditions_1
3D HNCO15N-13C-labeledisotropicsample_conditions_1
3D HNCA15N-13C-labeledisotropicsample_conditions_1
3D HNCACB15N-13C-labeledisotropicsample_conditions_1
3D CBCA(CO)NH15N-13C-labeledisotropicsample_conditions_1
3D C(CO)NH15N-13C-labeledisotropicsample_conditions_1
3D HBHA(CO)NH15N-13C-labeledisotropicsample_conditions_1
3D H(CCO)NH15N-13C-labeledisotropicsample_conditions_1
3D 1H-15N NOESY15N-13C-labeledisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAC35370 BAE20498 BAG62150
EMBL CAA36384
GB AAI37122 AAI37123 AAI67237 EAW54865 EAW54868
REF NP_001028400 NP_001296395 NP_112256 NP_852478 XP_001094788
SP P18614 P56199 Q3V3R4
TPG DAA17923
AlphaFold Q3V3R4 P56199 P18614

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks