BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17463

Title: Structure of the soluble methane monooxygenase regulatory protein B

Authors: Walters, Kylie; Gassner, George; Lippard, Stephen; Wagner, Gerhard

Citation: Walters, Kylie; Gassner, George; Lippard, Stephen; Wagner, Gerhard. "Structure of the soluble methane monooxygenase regulatory protein B"  Proc. Natl. Acad. Sci. U.S.A. 96, 7877-7882 (1999).

Assembly members:
MMOB, polymer, 141 residues, Formula weight is not available
MMOBHa, polymer, 512 residues, Formula weight is not available
MMOBHb, polymer, 384 residues, Formula weight is not available
MMOBHg, polymer, 162 residues, Formula weight is not available

Natural source:   Common Name: M. capsulatus   Taxonomy ID: 414   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Methylococcus capsulatus

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
MMOB: MSVNSNAYDAGIMGLKGKDF ADQFFADENQVVHESDTVVL VLKKSDEINTFIEEILLTDY KKNVNPTVNVEDRAGYWWIK ANGKIEVDCDEISELLGRQF NVYDFLVDVSSTIGRAYTLG NKFTITSELMGLDRKLEDYH A
MMOBHa: AANRAPTSVNAQEVHRWLQS FNWDFKNNRTKYATKYKMAN ETKEQFKLIAKEYARMEAVK DERQFGSLQVALTRLNAGVR VHPKWNETMKVVSNFLEVGE YNAIAATGMLWDSAQAAEQK NGYLAQVLDEIRHTHQCAYV NYYFAKNGQDPAGHNDARRT RTIGPLWKGMKRVFSDGFIS GDAVECSLNLQLVGEACFTN PLIVAVTEWAAANGDEITPT VFLSIETDELRHMANGYQTV VSIANDPASAKYLNTDLNNA FWTQQKYFTPVLGMLFEYGS KFKVEPWVKTWDRWVYEDWG GIWIGRLGKYGVESPRSLKD AKQDAYWAHHDLYLLAYALW PTGFFRLALPDQEEMEWFEA NYPGWYDHYGKIYEEWRARG CEDPSSGFIPLMWFIENNHP IYIDRVSQVPFCPSLAKGAS TLRVHEYNGEMHTFSDQWGE RMWLAEPERYECQNIFEQYE GRELSEVIAELHGLRSDGKT LIAQPHVRGDKLWTLDDIKR LNCVFKNPVKAF
MMOBHb: ERRRGLTDPEMAAVILKALP EAPLDGNNKMGYFVTPRWKR LTEYEALTVYAQPNADWIAG GLDWGDWTQKFHGGRPSWGN ETTELRTVDWFKHRDPLRRW HAPYVKDKAEEWRYTDRFLQ GYSADGQIRAMNPTWRTSSC NRYWGAFLFNEYGLFNAHSQ GAREALSDVTRVSLAFWGFD KIDIAQMIQLERGFLAKIVP GFDESTAVPKAEWTNGEVYK SARLAVEGLWQEVFDWNESA FSVHAVYDALFGQFVRREFF QRLAPRFGDNLTPFFINQAQ TYFQIAKQGVQDLYYNCLGD DPEFSDYNRTVMRNWTGKWL EPTIAALRDFMGLFAKLPAG TTDKEEITASLYRVVDDWIE DYASAIDFKADRDQIVKAVL AGLK
MMOBHg: LGIHSNDTRDAWVNKIAHVN TLEKAAEMLKQFRMDHTTPF RNSYELDNDYLWIEAKLEEK VAVLKARAFNEVDFRHKTAF GEDAKSVLDGTVAKMNAAKD KWEAEKIHIGFRQAYKPPIM PVNYFLDGERQLGTRLMELR NLNYYDTPLEELRKQRGVRV VH

Data sets:
Data typeCount
kinetic rates2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MMOB1
2MMOBHa2
3MMOBHb3
4MMOBHg4

Entities:

Entity 1, MMOB 141 residues - Formula weight is not available

1   METSERVALASNSERASNALATYRASPALA
2   GLYILEMETGLYLEULYSGLYLYSASPPHE
3   ALAASPGLNPHEPHEALAASPGLUASNGLN
4   VALVALHISGLUSERASPTHRVALVALLEU
5   VALLEULYSLYSSERASPGLUILEASNTHR
6   PHEILEGLUGLUILELEULEUTHRASPTYR
7   LYSLYSASNVALASNPROTHRVALASNVAL
8   GLUASPARGALAGLYTYRTRPTRPILELYS
9   ALAASNGLYLYSILEGLUVALASPCYSASP
10   GLUILESERGLULEULEUGLYARGGLNPHE
11   ASNVALTYRASPPHELEUVALASPVALSER
12   SERTHRILEGLYARGALATYRTHRLEUGLY
13   ASNLYSPHETHRILETHRSERGLULEUMET
14   GLYLEUASPARGLYSLEUGLUASPTYRHIS
15   ALA

Entity 2, MMOBHa 512 residues - Formula weight is not available

1   ALAALAASNARGALAPROTHRSERVALASN
2   ALAGLNGLUVALHISARGTRPLEUGLNSER
3   PHEASNTRPASPPHELYSASNASNARGTHR
4   LYSTYRALATHRLYSTYRLYSMETALAASN
5   GLUTHRLYSGLUGLNPHELYSLEUILEALA
6   LYSGLUTYRALAARGMETGLUALAVALLYS
7   ASPGLUARGGLNPHEGLYSERLEUGLNVAL
8   ALALEUTHRARGLEUASNALAGLYVALARG
9   VALHISPROLYSTRPASNGLUTHRMETLYS
10   VALVALSERASNPHELEUGLUVALGLYGLU
11   TYRASNALAILEALAALATHRGLYMETLEU
12   TRPASPSERALAGLNALAALAGLUGLNLYS
13   ASNGLYTYRLEUALAGLNVALLEUASPGLU
14   ILEARGHISTHRHISGLNCYSALATYRVAL
15   ASNTYRTYRPHEALALYSASNGLYGLNASP
16   PROALAGLYHISASNASPALAARGARGTHR
17   ARGTHRILEGLYPROLEUTRPLYSGLYMET
18   LYSARGVALPHESERASPGLYPHEILESER
19   GLYASPALAVALGLUCYSSERLEUASNLEU
20   GLNLEUVALGLYGLUALACYSPHETHRASN
21   PROLEUILEVALALAVALTHRGLUTRPALA
22   ALAALAASNGLYASPGLUILETHRPROTHR
23   VALPHELEUSERILEGLUTHRASPGLULEU
24   ARGHISMETALAASNGLYTYRGLNTHRVAL
25   VALSERILEALAASNASPPROALASERALA
26   LYSTYRLEUASNTHRASPLEUASNASNALA
27   PHETRPTHRGLNGLNLYSTYRPHETHRPRO
28   VALLEUGLYMETLEUPHEGLUTYRGLYSER
29   LYSPHELYSVALGLUPROTRPVALLYSTHR
30   TRPASPARGTRPVALTYRGLUASPTRPGLY
31   GLYILETRPILEGLYARGLEUGLYLYSTYR
32   GLYVALGLUSERPROARGSERLEULYSASP
33   ALALYSGLNASPALATYRTRPALAHISHIS
34   ASPLEUTYRLEULEUALATYRALALEUTRP
35   PROTHRGLYPHEPHEARGLEUALALEUPRO
36   ASPGLNGLUGLUMETGLUTRPPHEGLUALA
37   ASNTYRPROGLYTRPTYRASPHISTYRGLY
38   LYSILETYRGLUGLUTRPARGALAARGGLY
39   CYSGLUASPPROSERSERGLYPHEILEPRO
40   LEUMETTRPPHEILEGLUASNASNHISPRO
41   ILETYRILEASPARGVALSERGLNVALPRO
42   PHECYSPROSERLEUALALYSGLYALASER
43   THRLEUARGVALHISGLUTYRASNGLYGLU
44   METHISTHRPHESERASPGLNTRPGLYGLU
45   ARGMETTRPLEUALAGLUPROGLUARGTYR
46   GLUCYSGLNASNILEPHEGLUGLNTYRGLU
47   GLYARGGLULEUSERGLUVALILEALAGLU
48   LEUHISGLYLEUARGSERASPGLYLYSTHR
49   LEUILEALAGLNPROHISVALARGGLYASP
50   LYSLEUTRPTHRLEUASPASPILELYSARG
51   LEUASNCYSVALPHELYSASNPROVALLYS
52   ALAPHE

Entity 3, MMOBHb 384 residues - Formula weight is not available

1   GLUARGARGARGGLYLEUTHRASPPROGLU
2   METALAALAVALILELEULYSALALEUPRO
3   GLUALAPROLEUASPGLYASNASNLYSMET
4   GLYTYRPHEVALTHRPROARGTRPLYSARG
5   LEUTHRGLUTYRGLUALALEUTHRVALTYR
6   ALAGLNPROASNALAASPTRPILEALAGLY
7   GLYLEUASPTRPGLYASPTRPTHRGLNLYS
8   PHEHISGLYGLYARGPROSERTRPGLYASN
9   GLUTHRTHRGLULEUARGTHRVALASPTRP
10   PHELYSHISARGASPPROLEUARGARGTRP
11   HISALAPROTYRVALLYSASPLYSALAGLU
12   GLUTRPARGTYRTHRASPARGPHELEUGLN
13   GLYTYRSERALAASPGLYGLNILEARGALA
14   METASNPROTHRTRPARGTHRSERSERCYS
15   ASNARGTYRTRPGLYALAPHELEUPHEASN
16   GLUTYRGLYLEUPHEASNALAHISSERGLN
17   GLYALAARGGLUALALEUSERASPVALTHR
18   ARGVALSERLEUALAPHETRPGLYPHEASP
19   LYSILEASPILEALAGLNMETILEGLNLEU
20   GLUARGGLYPHELEUALALYSILEVALPRO
21   GLYPHEASPGLUSERTHRALAVALPROLYS
22   ALAGLUTRPTHRASNGLYGLUVALTYRLYS
23   SERALAARGLEUALAVALGLUGLYLEUTRP
24   GLNGLUVALPHEASPTRPASNGLUSERALA
25   PHESERVALHISALAVALTYRASPALALEU
26   PHEGLYGLNPHEVALARGARGGLUPHEPHE
27   GLNARGLEUALAPROARGPHEGLYASPASN
28   LEUTHRPROPHEPHEILEASNGLNALAGLN
29   THRTYRPHEGLNILEALALYSGLNGLYVAL
30   GLNASPLEUTYRTYRASNCYSLEUGLYASP
31   ASPPROGLUPHESERASPTYRASNARGTHR
32   VALMETARGASNTRPTHRGLYLYSTRPLEU
33   GLUPROTHRILEALAALALEUARGASPPHE
34   METGLYLEUPHEALALYSLEUPROALAGLY
35   THRTHRASPLYSGLUGLUILETHRALASER
36   LEUTYRARGVALVALASPASPTRPILEGLU
37   ASPTYRALASERALAILEASPPHELYSALA
38   ASPARGASPGLNILEVALLYSALAVALLEU
39   ALAGLYLEULYS

Entity 4, MMOBHg 162 residues - Formula weight is not available

1   LEUGLYILEHISSERASNASPTHRARGASP
2   ALATRPVALASNLYSILEALAHISVALASN
3   THRLEUGLULYSALAALAGLUMETLEULYS
4   GLNPHEARGMETASPHISTHRTHRPROPHE
5   ARGASNSERTYRGLULEUASPASNASPTYR
6   LEUTRPILEGLUALALYSLEUGLUGLULYS
7   VALALAVALLEULYSALAARGALAPHEASN
8   GLUVALASPPHEARGHISLYSTHRALAPHE
9   GLYGLUASPALALYSSERVALLEUASPGLY
10   THRVALALALYSMETASNALAALALYSASP
11   LYSTRPGLUALAGLULYSILEHISILEGLY
12   PHEARGGLNALATYRLYSPROPROILEMET
13   PROVALASNTYRPHELEUASPGLYGLUARG
14   GLNLEUGLYTHRARGLEUMETGLULEUARG
15   ASNLEUASNTYRTYRASPTHRPROLEUGLU
16   GLULEUARGLYSGLNARGGLYVALARGVAL
17   VALHIS

Samples:

sample_1: sodium phosphate buffer 50 mM; Pefabloc 4 mM; MMOB, [U-15N], 0.2 mM; sodium azide 0.1%; MMOBHa0.02 – 0.1 mM; MMOBb0.02 – 0.1 mM; MMOBg0.02 – 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2

Software:

FELIX, Accelrys Software Inc. - processing

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Bruker DMX 600 MHz
  • Varian Unity 500 MHz
  • Varian INOVA 500 MHz
  • Varian UnityPlus 400 MHz

Related Database Links:

BMRB 4431
PDB
GB AAF04158 AAU92726 AAB62392 AAU92736 EAA20247 AAB62393 AAU92727 EAA20248 AAF04157 AAU92724
REF WP_010960484 WP_010960482 XP_728682 YP_113659 WP_010960483 XP_728683 YP_113660 WP_010960485 WP_017364983 YP_113663
SP P18797 P22869 P18798 P11987