BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17538

Title: Structure of the second WW domain from human YAP in complex with a human Smad1 derived peptide   PubMed: 21685363

Authors: Macias, Maria; Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan

Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code."  Genes Dev. 25, 1275-1288 (2011).

Assembly members:
second WW domain from human YAP, polymer, 38 residues, 3979.426 Da.
human Smad1 derived peptide, polymer, 12 residues, 1293.435 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
second WW domain from human YAP: GAMEGPLPDGWEQAMTQDGE IYYINHKNKTTSWLDPRL
human Smad1 derived peptide: TPPPAYLPPEDP

Data sets:
Data typeCount
13C chemical shifts64
15N chemical shifts30
1H chemical shifts227

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1second WW domain from human YAP1
2human Smad1 derived peptide2

Entities:

Entity 1, second WW domain from human YAP 38 residues - 3979.426 Da.

1   GLYALAMETGLUGLYPROLEUPROASPGLY
2   TRPGLUGLNALAMETTHRGLNASPGLYGLU
3   ILETYRTYRILEASNHISLYSASNLYSTHR
4   THRSERTRPLEUASPPROARGLEU

Entity 2, human Smad1 derived peptide 12 residues - 1293.435 Da.

1   THRPROPROPROALATYRLEUPROPROGLU
2   ASPPRO

Samples:

H: NEDD4LWW3 1 mM; SMAD3 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 2 mM; H2O 90%; D2O 10%

15N: NEDD4LWW3, [U-100% 15N], 1 mM; SMAD3 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 2 mM; H2O 90%; D2O 10%

15N13C: NEDD4LWW3, [U-100% 13C; U-100% 15N], 1 mM; SMAD3 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.420 M; pH: 7; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYHisotropicsample_conditions_1
2D 1H-1H TOCSYHisotropicsample_conditions_1
3D CBCA(CO)NH15N13Cisotropicsample_conditions_1
3D HNCACB15N13Cisotropicsample_conditions_1
2D 1H-15N HSQC15Nisotropicsample_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

XEASY, Bartels et al. - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 18498
PDB 2LAW 2LTV
GB ETE73276
REF XP_004709068 XP_005329160 XP_013215694 XP_013215695 XP_013215696