BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17665

Title: human alpha synuclein construct   PubMed: 22006323

Authors: Pochapsky, Thomas; Perovic, Iva

Citation: Wang, Wei; Perovic, Iva; Chittuluru, Johnathan; Kaganovich, Alice; Nguyen, Linh; Liao, Jingling; Auclair, Jared; Johnson, Derrick; Landeru, Anuradha; Simorellis, Alana; Ju, Shulin; Cookson, Mark; Asturias, Francisco; Agar, Jeffrey; Webb, Brian; Kang, Chulhee; Ringe, Dagmar; Petsko, Gregory; Pochapsky, Thomas; Hoang, Quyen. "A soluble -synuclein construct forms a dynamic tetramer."  Proc. Natl. Acad. Sci. U.S.A. 108, 17797-17802 (2011).

Assembly members:
aSyn, polymer, 150 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
aSyn: GPLGSPEFPGMDVFMKGLSK AKEGVVAAAEKTKQGVAEAA GKTKEGVLYVGSKTKEGVVH GVATVAEKTKEQVTNVGGAV VTGVTAVAQKTVEGAGSIAA ATGFVKKDQLGKNEEGAPQE GILEDMPVDPDNEAYEMPSE EGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts134
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha synuclein, chain 11
2alpha synuclein, chain 21
3alpha synuclein, chain 31
4alpha synuclein, chain 41

Entities:

Entity 1, alpha synuclein, chain 1 150 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROGLUPHEPROGLY
2   METASPVALPHEMETLYSGLYLEUSERLYS
3   ALALYSGLUGLYVALVALALAALAALAGLU
4   LYSTHRLYSGLNGLYVALALAGLUALAALA
5   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
6   GLYSERLYSTHRLYSGLUGLYVALVALHIS
7   GLYVALALATHRVALALAGLULYSTHRLYS
8   GLUGLNVALTHRASNVALGLYGLYALAVAL
9   VALTHRGLYVALTHRALAVALALAGLNLYS
10   THRVALGLUGLYALAGLYSERILEALAALA
11   ALATHRGLYPHEVALLYSLYSASPGLNLEU
12   GLYLYSASNGLUGLUGLYALAPROGLNGLU
13   GLYILELEUGLUASPMETPROVALASPPRO
14   ASPASNGLUALATYRGLUMETPROSERGLU
15   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: aSyn, [U-99% 13C; U-99% 15N], 0.5 mM; HEPES 100 mM; NaCl 150 mM; Glycerol 10%; n-octyl-beta-glucopyranoside (BOG) 0.1%; H2O 90%; D2O 10%

sample_2: aSyn, [U-99% 15N], 0.5 mM; HEPES 100 mM; NaCl 150 mM; Glycerol 10%; n-octyl-beta-glucopyranoside BOG 0.1%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
15N-filtered 2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAB29375 BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143