BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17764

Title: A partially folded structure of amyloid-beta(1 40) in an aqueous environment   PubMed: 21726530

Authors: Vivekanandan, Subramanian; Brender, Jeffrey; Lee, Shirley; Ramamoorthy, Ayyalusamy

Citation: Vivekanandan, Subramanian; Brender, Jeffrey; Lee, Shirley; Ramamoorthy, Ayyalusamy. "A partially folded structure of amyloid-beta(1-40) in an aqueous environment."  Biochem. Biophys. Res. Commun. 411, 312-316 (2011).

Assembly members:
Abeta, polymer, 40 residues, 4335.893 Da.

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Abeta: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta1

Entities:

Entity 1, Abeta 40 residues - 4335.893 Da.

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: potassium phosphate 20 mM; sodium chloride 50 mM; Ab1-40, [U-10% 13C; U-100% 15N], 77 uM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.07 M; pH: 7.3; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 11435 15775 17159 17186 17793 17794 17795 17796 18052 18127 18128 18129 18131 19009 19309 19393 25218 25289 25429 26508 26516
PDB
DBJ BAA22264 BAA84580 BAB71958 BAD51938 BAE01907
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GB AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014
SP P05067 P53601 P79307 P86906 Q28053
TPG DAA33655