BMRB Entry 18079

Title:
UNAC Tetraloops: To What Extent Can They Mimic GNRA Tetraloops
Deposition date:
2011-11-14
Original release date:
2012-05-22
Authors:
Zhao, Q.; Huang, H.; Nagaswamy, U.; Xia, Y.; Gao, X.; Fox, George
Citation:

Citation: Zhao, Qin; Huang, Hung-Chung; Nagaswamy, Uma; Xia, Youlin; Gao, Xiaolian; Fox, George. "UNAC tetraloops: to what extent do they mimic GNRA tetraloops?"  Biopolymers 97, 617-628 (2012).
PubMed: 22605553

Assembly members:

Assembly members:
5'-R(*GP*GP*AP*CP*CP*CP*GP*GP*CP*UP*AP*AP*CP*GP_*CP*UP*GP*GP*GP*UP*CP*C)-3'_, polymer, 22 residues, 7048.30396 Da.

Natural source:

Natural source:   Common Name: Haloarcula marismortui   Taxonomy ID: 2238   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haloarcula marismortui

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5'-R(*GP*GP*AP*CP*CP*CP*GP*GP*CP*UP*AP*AP*CP*GP_*CP*UP*GP*GP*GP*UP*CP*C)-3'_: XGACCCGGCUAACGCUGGGU CC

Data sets:
Data typeCount
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNAC1

Entities:

Entity 1, UNAC 22 residues - 7048.30396 Da.

1   GMPGACCCGGCU
2   AACGCUGGGU
3   CC

Samples:

sample_1: UNAC, [U-13C; U-15N], 0.3 mM; D2O 99.96%

sample_conditions_1: pH: 7.000; pressure: 1 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1solutionsample_conditions_1
DQF-COSYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, AutoDep - chemical shift assignment

CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 0 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

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