BMRB Entry 18764

Title:
Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity
Deposition date:
2012-10-04
Original release date:
2012-10-29
Authors:
Lapinaite, Audrone; Simon, B.; Carlomagno, T.
Citation:

Citation: Ballare, Cecilia; Lange, Martin; Lapinaite, Audrone; Martin, Gloria Mas; Morey, Lluis; Pascual, Gloria; Liefke, Robert; Simon, Bernd; Shi, Yang; Gozani, Or; Carlomagno, Teresa; Benitah, Salvador Aznar; Di Croce, Luciano. "Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity."  Nat. Struct. Mol. Biol. 19, 1257-1265 (2012).
PubMed: 23104054

Assembly members:

Assembly members:
PHD_FINGER_PROTEIN_19, polymer, 58 residues, 6714.6566 Da.
HISTONE_H3, polymer, 11 residues, 1258.5055 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PHD_FINGER_PROTEIN_19: SKLTEGQYVLCRWTDGLYYL GKIKRVSSSKQSCLVTFEDN SKYWVLWKDIQHAGVPGE
HISTONE_H3: ATGGVXKPHRY

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts59
1H chemical shifts447

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PHD FINGER PROTEIN 191
2HISTONE H32

Entities:

Entity 1, PHD FINGER PROTEIN 19 58 residues - 6714.6566 Da.

1   SERLYSLEUTHRGLUGLYGLNTYRVALLEU
2   CYSARGTRPTHRASPGLYLEUTYRTYRLEU
3   GLYLYSILELYSARGVALSERSERSERLYS
4   GLNSERCYSLEUVALTHRPHEGLUASPASN
5   SERLYSTYRTRPVALLEUTRPLYSASPILE
6   GLNHISALAGLYVALPROGLYGLU

Entity 2, HISTONE H3 11 residues - 1258.5055 Da.

1   ALATHRGLYGLYVALM3LLYSPROHISARG
2   TYR

Samples:

sample_1: PHD FINGER PROTEIN 19, [U-13C; U-15N], 0.1 – 0.6 mM; HISTONE H3, [U-13C; U-15N], 0.1 – 0.6 mM

sample_conditions_1: ionic strength: 200.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
TRIPLE RESONANCEsample_1solutionsample_conditions_1
NOESYsample_1solutionsample_conditions_1
EDITED-FILTEREDsample_1solutionsample_conditions_1
EDITED -FILTEREDsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMRView vany, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP PHF19_HUMAN H31_HUMAN
PDB
DBJ BAG53700 BAI45704
EMBL CAE45832
GB AAH22374 AAI08664 AAI25077 AAI25078 ADQ32204
REF NP_001009936 NP_001179644 NP_001273769 NP_001273772 NP_056466
SP Q5T6S3
TPG DAA26446
AlphaFold Q9UFS9 Q93081 Q5T6S3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks