BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19009

Title: 40-residue beta-amyloid fibril derived from Alzheimer's disease brain   PubMed: 24034249

Authors: Lu, Jun-Xia; Qiang, Wei; Meredith, Stephen; Yau, Wai-Ming; Schweiters, Charles; Tycko, Robert

Citation: Lu, Jun-Xia; Qiang, Wei; Yau, Wai-Ming; Schweiters, Charles; Meredith, Stephen; Tycko, Robert. "Molecular Structure of beta-Amyloid Fibrils in Alzheimer's Disease Brain Tissue"  Cell 154, 1257-1268 (2013).

Assembly members:
beta-amyloid_peptide, polymer, 40 residues, 4335.893 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
beta-amyloid_peptide: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta-amyloid_peptide_11
2beta-amyloid_peptide_21
3beta-amyloid_peptide_31
4beta-amyloid_peptide_41
5beta-amyloid_peptide_51
6beta-amyloid_peptide_61
7beta-amyloid_peptide_71
8beta-amyloid_peptide_81
9beta-amyloid_peptide_91

Entities:

Entity 1, beta-amyloid_peptide_1 40 residues - 4335.893 Da.

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: beta-amyloid peptide, selectively and uniformly labeled samples, 1 – 2 mg

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C with fpRFDRsample_1solidsample_conditions_1
2D and 3D NCACXsample_1solidsample_conditions_1
2D NCOCXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D NCACXsample_1solidsample_conditions_1
2D 13C-13C with spin diffusionsample_1solidsample_conditions_1
2D 13C-13C with RADsample_1solidsample_conditions_1
2D 13C-13C with PARsample_1solidsample_conditions_1
2D 15N-13C TEDORsample_1solidsample_conditions_1
2D 15N-13C TEDORsample_1solidsample_conditions_1
15N- and 13C-BAREsample_1solidsample_conditions_1
13C PITHIRDS-CTsample_1solidsample_conditions_1
2D 13C-13C with fpRFDRsample_1solidsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculations

NMR spectrometers:

  • Varian InfinityPlus 600 MHz
  • Varian Infinity 750 MHz
  • Varian InfinityPlus 400 MHz

Related Database Links:

UNP P86906.1