BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19957

Title: Assignment of DNA-MC1 protein complex   PubMed: 25212183

Authors: Loth, Karine; Landon, Celine; Paquet, Francoise

Citation: Loth, Karine; Landon, Celine; Paquet, Francoise. "Chemical shifts assignments of the archaeal MC1 protein and a strongly bent 15 base pairs DNA duplex in complex"  Biomol. NMR Assignments ., .-. (2014).

Assembly members:
MC1, polymer, 93 residues, Formula weight is not available
DNA1, polymer, 15 residues, Formula weight is not available
DNA2, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: E. Coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MC1: SNTRNFVLRDEDGNEHGVFT GKQPRQAALKAANRGSGTKA NPDIIRLRERGTKKVHVFKA WKEIVDAPKNRPAWMPEKIS KPFVKKERIEKLE
DNA1: AAAAACACACACCCA
DNA2: TGGGTGTGTGTTTTT

Data typeCount
13C chemical shifts78
15N chemical shifts103
1H chemical shifts873

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MC11
2DNA12
3DNA23

Entities:

Entity 1, MC1 93 residues - Formula weight is not available

1   SERASNTHRARGASNPHEVALLEUARGASP
2   GLUASPGLYASNGLUHISGLYVALPHETHR
3   GLYLYSGLNPROARGGLNALAALALEULYS
4   ALAALAASNARGGLYSERGLYTHRLYSALA
5   ASNPROASPILEILEARGLEUARGGLUARG
6   GLYTHRLYSLYSVALHISVALPHELYSALA
7   TRPLYSGLUILEVALASPALAPROLYSASN
8   ARGPROALATRPMETPROGLULYSILESER
9   LYSPROPHEVALLYSLYSGLUARGILEGLU
10   LYSLEUGLU

Entity 2, DNA1 15 residues - Formula weight is not available

1   DADADADADADCDADCDADC
2   DADCDCDCDA

Entity 3, DNA2 15 residues - Formula weight is not available

1   DTDGDGDGDTDGDTDGDTDG
2   DTDTDTDTDT

Samples:

sample_1: MC1, [U-15N], 1.18 mM; DNA1, [U-13C; U-15N], 1.18 mM; DNA2 1.18 mM; sodium phosphate 10 mM; sodium chloride 100 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance III HD 700 MHz

Related Database Links:

PDB
GB AKB13417 AKB15948 ALK04717
REF WP_048167444
SP P12770