BMRB Entry 20022

Title:
1H Chemical Shift Assignments for metastin analog, s5a
Deposition date:
2008-04-17
Original release date:
2011-02-14
Authors:
Jae il, Kim
Citation:

Citation: Lee, Ju Yeon; Moon, Jung Sun; Eu, Young-Jae; Lee, Chul Won; Yang, Sung-Tae; Lee, Seung Kyu; Jung, Hyun Ho; Kim, Ha Hyung; Rhim, Hyewhon; Seong, Jae Young; Kim, Jae Il. "Molecular interaction between kisspeptin decapeptide analogs and a lipid membrane"  Arch. Biochem. Biophys. 485, 109-114 (2009).
PubMed: 19275876

Assembly members:

Assembly members:
GPCR_ligand_metastin_analog, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GPCR_ligand_metastin_analog: YNWNAFGLRFX

Data sets:
Data typeCount
1H chemical shifts61
conformer coordinate sets1
coupling constants9

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1metastin analog1

Entities:

Entity 1, metastin analog 11 residues - Formula weight is not available

1   TYRASNTRPASNALAPHEGLYLEUARGPHE
2   NH2

Samples:

S5A: GPCR_ligand_metastin_analog 1 mM; DPC, [U-100% 2H], 100 mM

sample_conditions_1: pH: 3.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYS5Aisotropicsample_conditions_1
2D 1H-1H TOCSYS5Aisotropicsample_conditions_1
2D DQF-COSYS5Aisotropicsample_conditions_1

Software:

CNS, Brunger - chemical shift calculation

NMR spectrometers:

  • Bruker Avance 600 MHz