BMRB Entry 20085

Title:
Solution structure of SFT-L1
Deposition date:
2009-07-21
Original release date:
2011-03-21
Authors:
Mylne, Joshua; Daly, Norelle; Colgrave, Michelle; McCallum, Emily; Jones, Alun; Craik, David
Citation:

Citation: Mylne, Joshua; Colgrave, Michelle; Daly, Norelle; Chanson, Aurelie; Elliott, Alysha; McCallum, Emily; Jones, Alun; Craik, David. "Albumins and their processing machinery are hijacked for cyclic peptides in sunflower"  Nat. Chem. Biol. 7, 257-259 (2011).
PubMed: 21423169

Assembly members:

Assembly members:
SFT-L1, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: sunflowers   Taxonomy ID: 4232   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Helianthus Helianthus annuus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SFT-L1: GCIEGSPVCFPD

Data sets:
Data typeCount
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SFT-L11

Entities:

Entity 1, SFT-L1 12 residues - Formula weight is not available

1   GLYCYSILEGLUGLYSERPROVALCYSPHE
2   PROASP

Samples:

sample_1: SFT-L1 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.1, Bruker Biospin - collection

XEASY, Bartels et al. - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz