BMRB Entry 20121

Title:
Tubulysin conformation bound to tubulin
Deposition date:
2010-02-03
Original release date:
2010-07-26
Authors:
Kubicek, Karel; Grimm, Steffen; Orts, Julien; Sasse, Florenz; Carlomagno, Teresa
Citation:

Citation: Kubicek, Karel; Grimm, S. Kaspar; Orts, Julien; Sasse, Florenz; Carlomagno, Teresa. "The tubulin-bound structure of the antimitotic drug tubulysin."  Angew. Chem. Int. Ed. Engl. 49, 4809-4812 (2010).
PubMed: 20496362

Assembly members:

Assembly members:
tubulysin, polymer, 4 residues, 841 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tubulysin: XIXX

Data sets:
Data typeCount
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tubulysin1

Entities:

Entity 1, tubulysin 4 residues - 841 Da.

1   T23ILETUVTUT

Samples:

sample_1: tubulysin 0.5 mM; tubulin 0.01 mM; sodium phosphate 1.5 mM; calcium phosphate 1.5 mM; H2O 95%; DMSO 5%

sample_conditions_1: temperature: 298 K; pH: 7.0

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH v2.31, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz