BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25651

Title: Isolation and structural characterization of an active G-quadruplex motif from AGRO100

Authors: Chung, Wan Jun; Heddi, Brahim; Phan, Anh Tuan

Citation: Chung, Wan Jun; Heddi, Brahim; Phan, Anh Tuan. "Isolation and structural characterization of an active G-quadruplex motif from AGRO100"  Not known ., .-..

Assembly members:
DNA_(28-MER), polymer, 28 residues, 8872.726 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_(28-MER): TGGTGGTGGTTGTTGTGGTG GTGGTGGT

Data sets:
Data typeCount
1H chemical shifts247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (28-MER)1

Entities:

Entity 1, DNA (28-MER) 28 residues - 8872.726 Da.

1   DTDGDGDTDGDGDTDGDGDT
2   DTDGDTDTDGDTDGDGDTDG
3   DGDTDGDGDTDGDGDT

Samples:

sample_1: DNA (28-MER)1.2 – 2 mM; KPi 20 mM; KCl 70 mM; H20 90%; D20 10%

sample_2: DNA (28-MER), [U-2% 15N], 0.2 – 1 mM; KPi 20 mM; KCl 70 mM; H20 90%; D20 10%

sample_3: DNA (28-MER)1.2 – 2 mM; KPi 20 mM; KCl 70 mM; D20 100%

sample_4: DNA (28-MER), [U-100% 2H], 0.2 – 1 mM; KPi 20 mM; KCl 70 mM; D20 100%

sample_conditions_1: ionic strength: 90 mM; pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
1D 15N-Filteredsample_2isotropicsample_conditions_1
1D D-labeledsample_4isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - geometry optimization, refinement, structure solution

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement, structure solution

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links: