BMRB Entry 25932

Title:
Transmembrane Structure of the P441A Mutant of the Cytokine Receptor Common Subunit beta
Deposition date:
2015-12-21
Original release date:
2016-07-05
Authors:
Schmidt, Thomas; Ye, Feng; Situ, Alan; An, Woojin; Ginsberg, Mark; Ulmer, Tobias
Citation:

Citation: Schmidt, Thomas; Ye, Feng; Situ, Alan; An, Woojin; Ginsberg, Mark; Ulmer, Tobias. "A Conserved Ectodomain-Transmembrane Domain Linker Motif Tunes the Allosteric Regulation of Cell Surface Receptors"  J. Biol. Chem. 291, 17536-17546 (2016).
PubMed: 27365391

Assembly members:

Assembly members:
entity, polymer, 44 residues, 5120.289 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET44-GB3-bc(P441A)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GKRSWDTESVLAMWVLALIV IFLTIAVLLALRFCGIYGYR LRRK

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts41
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 44 residues - 5120.289 Da.

1   GLYLYSARGSERTRPASPTHRGLUSERVAL
2   LEUALAMETTRPVALLEUALALEUILEVAL
3   ILEPHELEUTHRILEALAVALLEULEUALA
4   LEUARGPHECYSGLYILETYRGLYTYRARG
5   LEUARGARGLYS

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N; 80% 2H], 0.5 mM; H2O 93%; D2O 6%; 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) 350 mM; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) 105 mM; tris(2-carboxyethyl)phosphine (TCEP) 10 mM; HEPES NaOH 25 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D quant J correlationsample_1anisotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks