BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25964

Title: Structure of human islet amyloid polypeptide in complex with an engineered binding protein   PubMed: 27641459

Authors: Mirecka, Ewa; Feuerstein, Sophie; Stoldt, Matthias; Willbold, Dieter; Hoyer, Wolfgang

Citation: Mirecka, Ewa; Feuerstein, Sophie; Gremer, Lothar; Schroder, Gunnar; Stoldt, Matthias; Willbold, Dieter; Hoyer, Wolfgang. "beta-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor"  Sci. Rep. 6, 33474-33474 (2016).

Assembly members:
ISLET_AMYLOID_POLYPEPTIDE, polymer, 37 residues, 3908.322 Da.
HI18, polymer, 59 residues, 6531.2314 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ISLET_AMYLOID_POLYPEPTIDE: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY
HI18: VDNKFNKEMESAGGEIVYLP NLNPDQLCAFIHSIHDDPSQ SANLLAEAKKLNDAQAPKW

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts121
1H chemical shifts565

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ISLET AMYLOID POLYPEPTIDE1
2HI18, 12
3HI18, 22

Entities:

Entity 1, ISLET AMYLOID POLYPEPTIDE 37 residues - 3908.322 Da.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Entity 2, HI18, 1 59 residues - 6531.2314 Da.

1   VALASPASNLYSPHEASNLYSGLUMETGLU
2   SERALAGLYGLYGLUILEVALTYRLEUPRO
3   ASNLEUASNPROASPGLNLEUCYSALAPHE
4   ILEHISSERILEHISASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYSTRP

Samples:

sample_1: ISLET AMYLOID POLYPEPTIDE, [U-13C; U-15N], 0.40 mM; HI18 0.96 mM; sodium phosphate 20 mM

sample_2: ISLET AMYLOID POLYPEPTIDE 0.48 mM; HI18, [U-13C; U-15N], 0.80 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
HNHAsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
1H-15N NOESYsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HCCH COSYsample_1solutionsample_conditions_1
1H-13C NOESYsample_1solutionsample_conditions_1
1H-15N HSQCsample_2solutionsample_conditions_1
HNCAsample_2solutionsample_conditions_1
HNHAsample_2solutionsample_conditions_1
HNCACBsample_2solutionsample_conditions_1
1H-15N NOESYsample_2solutionsample_conditions_1
HCCH COSYsample_2solutionsample_conditions_1
1H-13C NOESYsample_2solutionsample_conditions_1

Software:

CcpNmr Analysis v2.4, CCPN - chemical shift assignment

NMRPipe vv7.9.2013.021.23.09, NIH - chemical shift assignment

TALOS-N v4.12, NIH - chemical shift assignment

VnmrJ vv3.2, Varian - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 800 MHz
  • Varian VNMRS 900 MHz

Related Database Links:

UNP IAPP_HUMAN
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