BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25991

Title: 1H, 13C, and 15N Chemical Shift Assignments of HAdV E1ACR3 (E1A CR3)   PubMed: 27490777

Authors: Hosek, Tomas; Nogueira, Marcela; Calcada, Eduardo; Pagani, Talita; Salvi, Nicola; Felli, Isabella; Pierattelli, Roberta

Citation: Hosek, Tomas; Calcada, Eduardo; Nogueira, Marcela; Salvi, Nicola; Pagani, Talita; Felli, Isabella; Pierattelli, Roberta. "Structural and Dynamic Characterization of the Molecular Hub Early Region 1A (E1A) from Human Adenovirus"  Chemistry 22, 13010-13013 (2016).

Assembly members:
E1A-CR3, polymer, 55 residues, Formula weight is not available

Natural source:   Common Name: Human adenovirus 2   Taxonomy ID: 10515   Superkingdom: Viruses   Kingdom: not available   Genus/species: Mastadenovirus Human mastadenovirus C

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E1A-CR3: MDEEGEEFVLDYVEHPGHGC RSCHYHRRNTGDPDIMCSLC YMRTCGMFVYSPVSE

Data sets:
Data typeCount
13C chemical shifts191
15N chemical shifts50
1H chemical shifts287

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1E1A-CR31

Entities:

Entity 1, E1A-CR3 55 residues - Formula weight is not available

1   METASPGLUGLUGLYGLUGLUPHEVALLEU
2   ASPTYRVALGLUHISPROGLYHISGLYCYS
3   ARGSERCYSHISTYRHISARGARGASNTHR
4   GLYASPPROASPILEMETCYSSERLEUCYS
5   TYRMETARGTHRCYSGLYMETPHEVALTYR
6   SERPROVALSERGLU

Samples:

sample_1: E1A-CR3, [U-100% 13C; U-100% 15N], 0.07 mM; HEPES 10 mM; potassium chloride 50 mM; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: E1A-CR3, [U-100% 13C; U-100% 15N], 0.14 mM; HEPES 10 mM; potassium chloride 50 mM; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N BEST TROSYsample_1isotropicsample_conditions_1
3D BEST TROSY HNCOsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D BEST TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D BEST TROSY HNCACBsample_1isotropicsample_conditions_1
3D BEST TROSY HNCAsample_2isotropicsample_conditions_1
3D BEST TROSY HN(CO)CAsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.0 and 3.1, Bruker Biospin - collection

CcpNMR vv2.3.1, CCPN - chemical shift assignment, data analysis, peak picking

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 950 MHz
  • Bruker Avance 900 MHz

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