BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26054

Title: NMR structure of omega-agatoxin IVA in DPC micelles   PubMed: 27838299

Authors: Ryu, Jae Ha; Kim, Jae Il

Citation: Ryu, Jae Ha; Jung, Hoi Jong; Konishi, Shiro; Kim, Ha Hyung; Park, Zee-Yong; Kim, Jae Il. "Structure-activity relationships of omega-Agatoxin IVA in lipid membranes"  Biochem. Biophys. Res. Commun. 482, 170-175 (2017).

Assembly members:
entity, polymer, 49 residues, 5277.445 Da.

Natural source:   Common Name: spiders   Taxonomy ID: 6908   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Agelenopsis aperta

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GKKKCIAKDYGRCKWGGTPC CRGRGCICSIMGTNCECKPR LIMEGLGLA

Data sets:
Data typeCount
15N chemical shifts48
1H chemical shifts298

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 49 residues - 5277.445 Da.

1   GLYLYSLYSLYSCYSILEALALYSASPTYR
2   GLYARGCYSLYSTRPGLYGLYTHRPROCYS
3   CYSARGGLYARGGLYCYSILECYSSERILE
4   METGLYTHRASNCYSGLUCYSLYSPROARG
5   LEUILEMETGLUGLYLEUGLYLEUALA

Samples:

sample_1: entity mM; DPC, [U-100% 2H], 50 mM

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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