BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26774

Title: 1H, 13C, and 15N Resonance Assignments for the Ribosomal Protein S1 280-438 from Mycobacterium tuberculosis   PubMed: 28258550

Authors: Fu, Jinglin; Liao, Xinli; Huang, Biling; Lin, Donghai

Citation: Fu, Jinglin; Huang, Biling; Lin, Donghai; Liao, Xinli. "Chemical shift assignments of Ribosomal protein S1 from Mycobacterium tuberculosis"  Biomol NMR Assign ., .-. (2017).

Assembly members:
Mtb_ribosomal_S1, polymer, 168 residues, Formula weight is not available

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 83332   Superkingdom: not available   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Mtb_ribosomal_S1: MQEDPWRHFARTHAIGQIVP GKVTKLVPFGAFVRVEEGIE GLVHISELAERHVEVPDQVV AVGDDAMVKVIDIDLERRRI SLSLKQANEDYTEEFDPAKY GMADSYDEQGNYIFPEGFDA ETNEWLEGFEKQRAEWEARY AEAERRHKMHTAQMEKFAAA LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts662
15N chemical shifts157
1H chemical shifts1054

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S1-like domains1

Entities:

Entity 1, S1-like domains 168 residues - Formula weight is not available

1   METGLNGLUASPPROTRPARGHISPHEALA
2   ARGTHRHISALAILEGLYGLNILEVALPRO
3   GLYLYSVALTHRLYSLEUVALPROPHEGLY
4   ALAPHEVALARGVALGLUGLUGLYILEGLU
5   GLYLEUVALHISILESERGLULEUALAGLU
6   ARGHISVALGLUVALPROASPGLNVALVAL
7   ALAVALGLYASPASPALAMETVALLYSVAL
8   ILEASPILEASPLEUGLUARGARGARGILE
9   SERLEUSERLEULYSGLNALAASNGLUASP
10   TYRTHRGLUGLUPHEASPPROALALYSTYR
11   GLYMETALAASPSERTYRASPGLUGLNGLY
12   ASNTYRILEPHEPROGLUGLYPHEASPALA
13   GLUTHRASNGLUTRPLEUGLUGLYPHEGLU
14   LYSGLNARGALAGLUTRPGLUALAARGTYR
15   ALAGLUALAGLUARGARGHISLYSMETHIS
16   THRALAGLNMETGLULYSPHEALAALAALA
17   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Mtb ribosomal S1, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Na2HPO4 20 mM; NaCl 150 mM; PMSF 1 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CCPN vV2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

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