BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26804

Title: POTRA1-5 backbone amide chemical shift assignments   PubMed: 27989620

Authors: Warner, Lisa; Gatzeva-Topolova, Petia; Doerner, Pamela; Pardi, Arthur; Sousa, Marcelo

Citation: Warner, Lisa; Gatzeva-Topolova, Petia; Doerner, Pamela; Pardi, Arthur; Sousa, Marcelo. "Flexibility in the Periplasmic Domain of BamA Is Important for Function"  Structure 25, 94-106 (2017).

Assembly members:
BamA_POTRA1-5, polymer, 409 residues, Formula weight is not available

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BamA_POTRA1-5: GAMAEGFVVKDIHFEGLQRV AVGAALLSMPVRTGDTVNDE DISNTIRALFATGNFEDVRV LRDGDTLLVQVKERPTIASI TFSGNKSVKDDMLKQNLEAS GVRVGESLDRTTIADIEKGL EDFYYSVGKYSASVKAVVTP LPRNRVDLKLVFQEGVSAEI QQINIVGNHAFTTDELISHF QLRDEVPWWNVVGDRKYQKQ KLAGDLETLRSYYLDRGYAR FNIDSTQVSLTPDKKGIYVT VNITEGDQYKLSGVEVSGNL AGHSAEIEQLTKIEPGELYN GTKVTKMEDDIKKLLGRYGY AYPRVQSMPEINDADKTVKL RVNVDAGNRFYVRKIRFEGN DTSKDAVLRREMRQMEGAWL GSDLVDQGKERLNRLGFFET VDTDTQRVPGSPDQVDVVYK VKERNTGPG

Data sets:
Data typeCount
15N chemical shifts319
1H chemical shifts319
T1 relaxation values253
T1rho relaxation values250
heteronuclear NOE values256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1POTRA1-51

Entities:

Entity 1, POTRA1-5 409 residues - Formula weight is not available

1   GLYALAMETALAGLUGLYPHEVALVALLYS
2   ASPILEHISPHEGLUGLYLEUGLNARGVAL
3   ALAVALGLYALAALALEULEUSERMETPRO
4   VALARGTHRGLYASPTHRVALASNASPGLU
5   ASPILESERASNTHRILEARGALALEUPHE
6   ALATHRGLYASNPHEGLUASPVALARGVAL
7   LEUARGASPGLYASPTHRLEULEUVALGLN
8   VALLYSGLUARGPROTHRILEALASERILE
9   THRPHESERGLYASNLYSSERVALLYSASP
10   ASPMETLEULYSGLNASNLEUGLUALASER
11   GLYVALARGVALGLYGLUSERLEUASPARG
12   THRTHRILEALAASPILEGLULYSGLYLEU
13   GLUASPPHETYRTYRSERVALGLYLYSTYR
14   SERALASERVALLYSALAVALVALTHRPRO
15   LEUPROARGASNARGVALASPLEULYSLEU
16   VALPHEGLNGLUGLYVALSERALAGLUILE
17   GLNGLNILEASNILEVALGLYASNHISALA
18   PHETHRTHRASPGLULEUILESERHISPHE
19   GLNLEUARGASPGLUVALPROTRPTRPASN
20   VALVALGLYASPARGLYSTYRGLNLYSGLN
21   LYSLEUALAGLYASPLEUGLUTHRLEUARG
22   SERTYRTYRLEUASPARGGLYTYRALAARG
23   PHEASNILEASPSERTHRGLNVALSERLEU
24   THRPROASPLYSLYSGLYILETYRVALTHR
25   VALASNILETHRGLUGLYASPGLNTYRLYS
26   LEUSERGLYVALGLUVALSERGLYASNLEU
27   ALAGLYHISSERALAGLUILEGLUGLNLEU
28   THRLYSILEGLUPROGLYGLULEUTYRASN
29   GLYTHRLYSVALTHRLYSMETGLUASPASP
30   ILELYSLYSLEULEUGLYARGTYRGLYTYR
31   ALATYRPROARGVALGLNSERMETPROGLU
32   ILEASNASPALAASPLYSTHRVALLYSLEU
33   ARGVALASNVALASPALAGLYASNARGPHE
34   TYRVALARGLYSILEARGPHEGLUGLYASN
35   ASPTHRSERLYSASPALAVALLEUARGARG
36   GLUMETARGGLNMETGLUGLYALATRPLEU
37   GLYSERASPLEUVALASPGLNGLYLYSGLU
38   ARGLEUASNARGLEUGLYPHEPHEGLUTHR
39   VALASPTHRASPTHRGLNARGVALPROGLY
40   SERPROASPGLNVALASPVALVALTYRLYS
41   VALLYSGLUARGASNTHRGLYPROGLY

Samples:

sample_1: BamA POTRA1-5, [U-13C; U-15N; U-2H], 1 mM; D2O 10%; MES 50 mM; sodium chloride 50 mM; EDTA 0.1 mM; sodium azide 0.01%; TSP 0.14 mM; HALT protease inhibitor 1 tablet/100mL

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links: