BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26853

Title: Identification and structural characterization of LytU   PubMed: 28729697

Authors: Tossavainen, Helena; Raulinaitis, Vytas; Permi, Perttu

Citation: Raulinaitis, Vytas; Tossavainen, Helena; Aitio, Olli; Juuti, Jarmo; Hiramatsu, Keiichi; Kontinen, Vesa; Permi, Perttu. "Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family"  Sci. Rep. 7, 6020-6020 (2017).

Assembly members:
LytU, polymer, 145 residues, Formula weight is not available

Natural source:   Common Name: firmicutes   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LytU: GSKWEDFFRGSRITETFGKY QHSPFDGKHYGIDFALPKGT PIKAPTNGKVTRIFNNELGG KVLQIAEDNGEYHQWYLHLD KYNVKVGDRVKAGDIIAYSG NTGIQTTGAHLHFQRMKGGV GNAYAEDPKPFIDQLPDGER SLYDL

Data sets:
Data typeCount
13C chemical shifts467
15N chemical shifts136
1H chemical shifts933

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LytU1

Entities:

Entity 1, LytU 145 residues - Formula weight is not available

1   GLYSERLYSTRPGLUASPPHEPHEARGGLY
2   SERARGILETHRGLUTHRPHEGLYLYSTYR
3   GLNHISSERPROPHEASPGLYLYSHISTYR
4   GLYILEASPPHEALALEUPROLYSGLYTHR
5   PROILELYSALAPROTHRASNGLYLYSVAL
6   THRARGILEPHEASNASNGLULEUGLYGLY
7   LYSVALLEUGLNILEALAGLUASPASNGLY
8   GLUTYRHISGLNTRPTYRLEUHISLEUASP
9   LYSTYRASNVALLYSVALGLYASPARGVAL
10   LYSALAGLYASPILEILEALATYRSERGLY
11   ASNTHRGLYILEGLNTHRTHRGLYALAHIS
12   LEUHISPHEGLNARGMETLYSGLYGLYVAL
13   GLYASNALATYRALAGLUASPPROLYSPRO
14   PHEILEASPGLNLEUPROASPGLYGLUARG
15   SERLEUTYRASPLEU

Samples:

sample_1: LytU, [U-13C; U-15N], 0.5 mM; Bis-Tris 20 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

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