BMRB Entry 26910

Title:
13C and 15N chemical shift assignment of the Y21M mutation of fd filamentous phage
Deposition date:
2016-09-28
Original release date:
2017-07-19
Authors:
Goldbourt, Amir; Abramov, Gili
Citation:

Citation: Abramov, Gili; Shaharabani, Rona; Morag, Omry; Avinery, Ram; Haimovich, Anat; Oz, Inbal; Beck, Roy; Goldbourt, Amir. "Structural Effects of Single Mutations in a Filamentous Viral Capsid Across Multiple Length Scales"  Biomacromolecules 18, 2258-2266 (2017).
PubMed: 28657731

Assembly members:

Assembly members:
fd-y21m_phage, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fd-y21m_phage: AEGDDPAKAAFDSLQASATE MIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1y21m protein coat monomer1

Entities:

Entity 1, y21m protein coat monomer 50 residues - Formula weight is not available

1   ALAGLUGLYASPASPPROALALYSALAALA
2   PHEASPSERLEUGLNALASERALATHRGLU
3   METILEGLYTYRALATRPALAMETVALVAL
4   VALILEVALGLYALATHRILEGLYILELYS
5   LEUPHELYSLYSPHETHRSERLYSALASER

Samples:

sample_1: fd-y21m phage, [U-13C; U-15N], 7 mg

sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1anisotropicsample_conditions_1
3D NCACXsample_1anisotropicsample_conditions_1
3D NCOCXsample_1anisotropicsample_conditions_1

Software:

nmrpipe, Delaglio - processing

NMR spectrometers:

  • Bruker Avance 600 MHz