BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26978

Title: MeCP2 bound to methylated DNA   PubMed: 28450074

Authors: Sperlazza, M. Jeannette; David, Williams

Citation: Sperlazza, M. Jeannette; Bilinovich, Stephanie; Sinanan, Leander; Javier, Fatima; Williams, David. "Structural Basis of MeCP2 Distribution on Non-CpG Methylated and Hydroxymethylated DNA"  J. Mol. Biol. 429, 1581-1594 (2017).

Assembly members:
MeCP2_MBD, polymer, 89 residues, Formula weight is not available
BDNF_DNA, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MeCP2_MBD: MASASPKQRRSIIRDRGPMY DDPTLPEGWTRKLKQRKSGR SAGKYDVYLINPQGKAFRSK VELIAYFEKVGDTSLDPNDF DFTVTGRGS
BDNF_DNA: TAGAAGAATTCXGTTCCAG

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts72
1H chemical shifts305

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MeCP21
2DNA, chain 12
3DNA, chain 22

Entities:

Entity 1, MeCP2 89 residues - Formula weight is not available

1   METALASERALASERPROLYSGLNARGARG
2   SERILEILEARGASPARGGLYPROMETTYR
3   ASPASPPROTHRLEUPROGLUGLYTRPTHR
4   ARGLYSLEULYSGLNARGLYSSERGLYARG
5   SERALAGLYLYSTYRASPVALTYRLEUILE
6   ASNPROGLNGLYLYSALAPHEARGSERLYS
7   VALGLULEUILEALATYRPHEGLULYSVAL
8   GLYASPTHRSERLEUASPPROASNASPPHE
9   ASPPHETHRVALTHRGLYARGGLYSER

Entity 2, DNA, chain 1 19 residues - Formula weight is not available

double stranded DNA X = methyl-cytosine

1   DTDADGDADADGDADADTDT
2   DC1MCDGDTDTDCDCDADG

Samples:

sample_1: MeCP2 MBD, [U-99% 13C; U-99% 15N], 0.75 mM; BDNF DNA 0.75 mM; sodium phosphate 10 mM; sodium azide 0.02 % w/v; DTT 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links: