BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26984

Title: Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein   PubMed: 28739907

Authors: Sekar, Giridhar; Cowburn, David

Citation: Stevens, Adam; Sekar, Giridhar; Shah, Neel; Mostafavi, Ana; Cowburn, David; Muir, Tom. "A promiscuous split intein with expanded protein engineering applications"  Proc. Natl. Acad. Sci. U. S. A. 114, 8538-8543 (2017).

Assembly members:
Npu_DnaE, polymer, 144 residues, 16403.5412 Da.

Natural source:   Common Name: Nostoc punctiforme   Taxonomy ID: 272131   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc punctiforme

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Npu_DnaE: AEYALSYETEILTVEYGLLP IGKIVEKRIECTVYSVDNNG NIYTQPVAQWHDRGEQEVFE YCLEDGSLIRATKDHKFMTV DGQMLPIDEIFERELDLMRV DNLPNMIKIATRKYLGKQNV YDIGVGEPHNFALKNGFIAS ACGN

Data sets:
Data typeCount
13C chemical shifts412
15N chemical shifts141
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Fused Npu DnaE GEP loop mutant1

Entities:

Entity 1, Fused Npu DnaE GEP loop mutant 144 residues - 16403.5412 Da.

1   ALAGLUTYRALALEUSERTYRGLUTHRGLU
2   ILELEUTHRVALGLUTYRGLYLEULEUPRO
3   ILEGLYLYSILEVALGLULYSARGILEGLU
4   CYSTHRVALTYRSERVALASPASNASNGLY
5   ASNILETYRTHRGLNPROVALALAGLNTRP
6   HISASPARGGLYGLUGLNGLUVALPHEGLU
7   TYRCYSLEUGLUASPGLYSERLEUILEARG
8   ALATHRLYSASPHISLYSPHEMETTHRVAL
9   ASPGLYGLNMETLEUPROILEASPGLUILE
10   PHEGLUARGGLULEUASPLEUMETARGVAL
11   ASPASNLEUPROASNMETILELYSILEALA
12   THRARGLYSTYRLEUGLYLYSGLNASNVAL
13   TYRASPILEGLYVALGLYGLUPROHISASN
14   PHEALALEULYSASNGLYPHEILEALASER
15   ALACYSGLYASN

Samples:

sample_1: sodium phosphate 20 mM; TCEP 2 mM; Npu DnaE, [U-99% 13C; U-99% 15N], 400 mM

CondSet1: pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicCondSet1
3D HNCACBsample_1isotropicCondSet1
3D CBCA(CO)NHsample_1isotropicCondSet1
3D HN(CA)COsample_1isotropicCondSet1
3D HNCOsample_1isotropicCondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - Spectrum processing, chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

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