BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27030

Title: Chemical shift assignments of DANCER-0, a dynamic pentamutant of the B1 domain of streptococcal protein G (GB1)

Authors: Damry, Adam; Davey, James; Goto, Natalie; Chica, Roberto

Citation: Davey, James; Damry, Adam; Goto, Natalie; Chica, Roberto. "Design of conformational exchange into proteins via energy landscape engineering"  Not known ., .-..

Assembly members:
DANCER-0, polymer, 64 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus sp. GX7805   Taxonomy ID: 1325   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. GX7805

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DANCER-0: MHHHHHHGMTFKAIINGKTL KGETTTEAVDAATAEKVFKQ YFNDNGIDGEWTYDDATKTF TVTE

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts47
1H chemical shifts305

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DANCER-01

Entities:

Entity 1, DANCER-0 64 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYMETTHR
2   PHELYSALAILEILEASNGLYLYSTHRLEU
3   LYSGLYGLUTHRTHRTHRGLUALAVALASP
4   ALAALATHRALAGLULYSVALPHELYSGLN
5   TYRPHEASNASPASNGLYILEASPGLYGLU
6   TRPTHRTYRASPASPALATHRLYSTHRPHE
7   THRVALTHRGLU

Samples:

sample_1: DANCER-0, [U-99% 13C; U-98% 15N], 1 mM; sodium phosphate 10 mM

sample_2: DANCER-0, [U-99% 13C; U-98% 15N], 1 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links: