BMRB Entry 27065

Title:
13C, 15N chemical shift assignments of MAK33 VL S20N amyloid fibrils
Deposition date:
2017-04-06
Original release date:
2017-04-27
Authors:
Hora, Manuel; Sarkar, Riddhiman; Morris, Vanessa; Xue, Kai; Prade, Elke; Harding, Emma; Buchner, Johannes; Reif, Bernd
Citation:

Citation: Hora, Manuel; Sarkar, Riddhiman; Morris, Vanessa; Xue, Kai; Prade, Elke; Harding, Emma; Buchner, Johannes; Reif, Bernd. "MAK33 antibody light chain amyloid fibrils are similar to oligomeric precursors."  PLoS ONE 12, e0181799-e0181799 (2017).
PubMed: 28746363

Assembly members:

Assembly members:
MAK33_VL, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 28a

Data sets:
Data typeCount
13C chemical shifts111
15N chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAK33 VL amyloid fibril1

Entities:

Entity 1, MAK33 VL amyloid fibril 108 residues - Formula weight is not available

this is the variable domain of an antibody light chain

1   ASPILEVALLEUTHRGLNSERPROALATHR
2   LEUSERVALTHRPROGLYASPSERVALASN
3   LEUSERCYSARGALASERGLNSERILESER
4   ASNASNLEUHISTRPTYRGLNGLNLYSSER
5   HISGLUSERPROARGLEULEUILELYSTYR
6   ALASERGLNSERILESERGLYILEPROSER
7   ARGPHESERGLYSERGLYSERGLYTHRASP
8   PHETHRLEUSERILEASNSERVALGLUTHR
9   GLUASPPHEGLYMETTYRPHECYSGLNGLN
10   SERASNSERTRPPROLEUTHRPHEGLYALA
11   GLYTHRLYSLEUGLULEULYSARG

Samples:

sample_1: MAK33 VL, [U-13C; U-15N], mM; acetic acid 25 mM; phosphoric acid 25 mM; potassium chloride 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 175 mM; pH: 2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D PDSD (50 ms)sample_1solidsample_conditions_1
2D NCACXsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
2D NCOCXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D NCACBsample_1solidsample_conditions_1
3D NCACBsample_1solidsample_conditions_1
2D NcoCACBsample_1solidsample_conditions_1
3D NCOCAsample_1solidsample_conditions_1
3D CANCOsample_1solidsample_conditions_1
2D 15N INEPTsample_1solidsample_conditions_1

Software:

CcpNMR_Analysis v2, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz