BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27079

Title: Backbone Resonance Assignment of the BCL6-BTB/POZ Domain   PubMed: 28929458

Authors: Muskett, Frederick; Lin, Li-Ying; Wagner, Simon; Schwabe, John

Citation: Lin, Li-Ying; Evans, S; Fairall, L; Schwabe, John; Wagner, Simon; Muskett, Frederick. "Backbone resonance assignment of the BCL6-BTB/POZ domain"  Biomol. NMR Assignments ., .-. (2017).

Assembly members:
bl6, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
bl6: GASQIQFTRHASDVLLNLNR LRSRDILTDVVIVVSREQFR AHKTVLMACSGLFYSIFTDQ LKRNLSVINLDPEINPEGFN ILLDFMYTSRLNLREGNIMA VMATAMYLQMEHVVDTCRKF IKAS

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts217
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bcl61

Entities:

Entity 1, bcl6 124 residues - Formula weight is not available

1   GLYALASERGLNILEGLNPHETHRARGHIS
2   ALASERASPVALLEULEUASNLEUASNARG
3   LEUARGSERARGASPILELEUTHRASPVAL
4   VALILEVALVALSERARGGLUGLNPHEARG
5   ALAHISLYSTHRVALLEUMETALACYSSER
6   GLYLEUPHETYRSERILEPHETHRASPGLN
7   LEULYSARGASNLEUSERVALILEASNLEU
8   ASPPROGLUILEASNPROGLUGLYPHEASN
9   ILELEULEUASPPHEMETTYRTHRSERARG
10   LEUASNLEUARGGLUGLYASNILEMETALA
11   VALMETALATHRALAMETTYRLEUGLNMET
12   GLUHISVALVALASPTHRCYSARGLYSPHE
13   ILELYSALASER

Samples:

sample_1: bl6, [U-100% 13C; U-100% 15N; U-80% 2H], 200 uM; sodium phosphate 50 mM; L-Arginine 50 mM; L-Glutamic acid 50 mM; TCEP 1 mM; sodium azide 3 mM

sample_2: bl6, [U-100% 13C; U-100% 15N; U-80% 2H], 200 uM; sodium phosphate 50 mM; TCEP 1 mM; sodium azide 3 mM; sodium chloride 300 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 400 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

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