BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27080

Title: Backbone resonance assignment of the Human Uracil DNA Glycosylase-2   PubMed: 28940147

Authors: Kara, Hesna; Ponchon, Luc; Bouaziz, Serge

Citation: Kara, Hesna; Ponchon, Luc; Bouaziz, Serge. "Backbone resonance assignment of the Human Uracil DNA Glycosylase-2"  Biomol. NMR Assignments ., .-. (2017).

Assembly members:
UNG2, polymer, 248 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UNG2: MGSSHHHHHHSSGLVPRGSH MENLYFQGFGESWKKHLSGE FGKPYFIKLMGFVAEERKHY TVYPPPHQVFTWTQMCDIKD VKVVILGQDPYHGPNQAHGL CFSVQRPVPPPPSLENIYKE LSTDIEDFVHPGHGDLSGWA KQGVLLLNAVLTVRAHQANS HKERGWEQFTDAVVSWLNQN SNGLVFLLWGSYAQKKGSAI DRKRHHVLQTAHPSPLSVYR GFFGCRHFSKTNELLQKSGK KPIDWKEL

Data sets:
Data typeCount
13C chemical shifts692
15N chemical shifts231
1H chemical shifts231

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNG21

Entities:

Entity 1, UNG2 248 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLUASNLEUTYRPHEGLNGLYPHEGLY
4   GLUSERTRPLYSLYSHISLEUSERGLYGLU
5   PHEGLYLYSPROTYRPHEILELYSLEUMET
6   GLYPHEVALALAGLUGLUARGLYSHISTYR
7   THRVALTYRPROPROPROHISGLNVALPHE
8   THRTRPTHRGLNMETCYSASPILELYSASP
9   VALLYSVALVALILELEUGLYGLNASPPRO
10   TYRHISGLYPROASNGLNALAHISGLYLEU
11   CYSPHESERVALGLNARGPROVALPROPRO
12   PROPROSERLEUGLUASNILETYRLYSGLU
13   LEUSERTHRASPILEGLUASPPHEVALHIS
14   PROGLYHISGLYASPLEUSERGLYTRPALA
15   LYSGLNGLYVALLEULEULEUASNALAVAL
16   LEUTHRVALARGALAHISGLNALAASNSER
17   HISLYSGLUARGGLYTRPGLUGLNPHETHR
18   ASPALAVALVALSERTRPLEUASNGLNASN
19   SERASNGLYLEUVALPHELEULEUTRPGLY
20   SERTYRALAGLNLYSLYSGLYSERALAILE
21   ASPARGLYSARGHISHISVALLEUGLNTHR
22   ALAHISPROSERPROLEUSERVALTYRARG
23   GLYPHEPHEGLYCYSARGHISPHESERLYS
24   THRASNGLULEULEUGLNLYSSERGLYLYS
25   LYSPROILEASPTRPLYSGLULEU

Samples:

sample_1: UNG2, [U-100% 13C; U-100% 15N], 0.4 mM; potassium phosphate 40 mM; DTT 5 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker - acquisition, processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

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