BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27104

Title: Steroid receptor coactivator-2 (SRC-2/NCoA-2/GRIP1/TIF2) receptor interaction domain (RID) backbone NMR chemical shift assignments   PubMed: 28890360

Authors: de Vera, Ian

Citation: de Vera, Ian Mitchelle; Zheng, Jie; Novick, Scott; Shang, Jinsai; Hughes, Travis; Brust, Richard; Munoz-Tello, Paola; Gardner, William; Marciano, David; Kong, Xiangming; Griffin, Patrick; Kojetin, Douglas. "Synergistic regulation of coregulator/nuclear receptor interaction by ligand and DNA"  Structure ., 30253-30258 (2017).

Assembly members:
SRC-2_RID, polymer, 235 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SRC-2_RID: MGSSHHHHHHSSGLVPRGSH MDYKDDDDKERADGQSRLHD SKGQTKLLQLLTTKSDQMEP SPLASSLSDTNKDSTGSLPG SGSTHGTSLKEKHKILHRLL QDSSSPVDLAKLTAEATGKD LSQESSSTAPGSEVTIKQEP VSPKKKENALLRYLLDKDDT KDIGLPEITPKLERLDSKTD PASNTKLIAMKTEKEEMSFE PGDQPGSELDNLEEILDDLQ NSQLPQLFPDTRPGA

Data sets:
Data typeCount
13C chemical shifts642
15N chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SRC-2 RID1

Entities:

Entity 1, SRC-2 RID 235 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASPTYRLYSASPASPASPASPLYSGLU
4   ARGALAASPGLYGLNSERARGLEUHISASP
5   SERLYSGLYGLNTHRLYSLEULEUGLNLEU
6   LEUTHRTHRLYSSERASPGLNMETGLUPRO
7   SERPROLEUALASERSERLEUSERASPTHR
8   ASNLYSASPSERTHRGLYSERLEUPROGLY
9   SERGLYSERTHRHISGLYTHRSERLEULYS
10   GLULYSHISLYSILELEUHISARGLEULEU
11   GLNASPSERSERSERPROVALASPLEUALA
12   LYSLEUTHRALAGLUALATHRGLYLYSASP
13   LEUSERGLNGLUSERSERSERTHRALAPRO
14   GLYSERGLUVALTHRILELYSGLNGLUPRO
15   VALSERPROLYSLYSLYSGLUASNALALEU
16   LEUARGTYRLEULEUASPLYSASPASPTHR
17   LYSASPILEGLYLEUPROGLUILETHRPRO
18   LYSLEUGLUARGLEUASPSERLYSTHRASP
19   PROALASERASNTHRLYSLEUILEALAMET
20   LYSTHRGLULYSGLUGLUMETSERPHEGLU
21   PROGLYASPGLNPROGLYSERGLULEUASP
22   ASNLEUGLUGLUILELEUASPASPLEUGLN
23   ASNSERGLNLEUPROGLNLEUPHEPROASP
24   THRARGPROGLYALA

Samples:

sample_1: SRC-2 RID, [U-100% 13C; U-100% 15N], 400 uM; KPO4 20 mM; KCl 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D (HaCa)CONsample_1isotropicsample_conditions_1
2D (HCaCO)N(CO)Casample_1isotropicsample_conditions_1
3D (H)CbCaCONsample_1isotropicsample_conditions_1
3D (H)CbCaNCOsample_1isotropicsample_conditions_1
3D (H)CaNCaCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

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