BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27108

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SH3 domain of Noxa1   PubMed: 28625920

Authors: Shrestha, Pravesh

Citation: Shrestha, Pravesh; Yun, Ji-Hye; Ko, Yoon-Joo; Kim, Myeongkyu; Bae, Yun Soo; Lee, Weontae. "C-terminal tail of NADPH oxidase organizer 1 (Noxo1) mediates interaction with NADPH oxidase activator (Noxa1) in the NOX1 complex"  Biochem. Biophys. Res. Commun. 490, 594-600 (2017).

Assembly members:
NOX1_activator, polymer, 64 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NOX1_activator: QRPVLYQVVAQHSYSAQGPE DLGFRQGDTVDVLCEVDQAW LEGHCDGRIGIFPKCVVPAG PRMS

Data sets:
Data typeCount
13C chemical shifts116
15N chemical shifts57
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NOX1 ativator1

Entities:

Entity 1, NOX1 ativator 64 residues - Formula weight is not available

1   GLNARGPROVALLEUTYRGLNVALVALALA
2   GLNHISSERTYRSERALAGLNGLYPROGLU
3   ASPLEUGLYPHEARGGLNGLYASPTHRVAL
4   ASPVALLEUCYSGLUVALASPGLNALATRP
5   LEUGLUGLYHISCYSASPGLYARGILEGLY
6   ILEPHEPROLYSCYSVALVALPROALAGLY
7   PROARGMETSER

Samples:

sample_1: NOX1 ativator, na, mM; HEPES 10 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.01%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 850 MHz

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