BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27140

Title: 3Jcgn & 3Jcgc' scalar couplings for CUG-BP2 RRM3 wild type   PubMed: 28935965

Authors: Damberger, Fred

Citation: Diarra Dit Konte, Nana; Krepl, Miroslav; Damberger, Fred; Ripin, Nina; Duss, Olivier; Sponer, Jiri; Allain, Frederic H-T. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination"  Nat. Commun. 8, 654-654 (2017).

Assembly members:
CUG_BP2_RRM3, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CUG_BP2_RRM3: MGSSHHHHHHSSGLVPRGSH MQKEGPEGANLFIYHLPQEF GDQDILQMFMPFGNVISAKV FIDKQTNLSKCFGFVSYDNP VSAQAAIQAMNGFQIGMKRL KVQLKRSKNDSKPW

Data sets:
Data typeCount
coupling constants20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM31

Entities:

Entity 1, RRM3 114 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLNLYSGLUGLYPROGLUGLYALAASN
4   LEUPHEILETYRHISLEUPROGLNGLUPHE
5   GLYASPGLNASPILELEUGLNMETPHEMET
6   PROPHEGLYASNVALILESERALALYSVAL
7   PHEILEASPLYSGLNTHRASNLEUSERLYS
8   CYSPHEGLYPHEVALSERTYRASPASNPRO
9   VALSERALAGLNALAALAILEGLNALAMET
10   ASNGLYPHEGLNILEGLYMETLYSARGLEU
11   LYSVALGLNLEULYSARGSERLYSASNASP
12   SERLYSPROTRP

Samples:

sample_1: CUG BP2 RRM3, [U-100% 13C; U-100% 15N], 0.65 mM; D2O, [U-100% 2H], 5 % v/v; H2O 95 % v/v; sodium chloride 10 mM; potassium phosphate 20 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
spin-echo difference constant time 15N-HSQCsample_1isotropicsample_conditions_1
spin-echo difference constant time HN(CO)CGsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 600 MHz

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